GAP43 recombinant protein

GAP43 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121159

• Gene Names: GAP43; B-50; PP46
• Purity: >96% as determined by SDS-PAGE
• Synonyms: GAP43; GAP43 Protein; Human; Recombinant (His Tag); Human GAP43/Neuromodulin Protein (His Tag); B-50 Protein; PP46 Protein; growth associated protein 43; GAP43 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala238

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human GAP43 (P17677)(Met 1-Ala 238) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GAP43 recombinant protein

Background: Neuromodulin, also known as Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-5, pp46 and GAP43, is a cell membrane protein which belongs to theneuromodulin family. Neuromodulin/GAP43 contains oneIQ domain. Neuromodulin/GAP43 is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Neuromodulin/GAP43 is involved in neurite outgrowth, a crucial process for the differentiation of neurons. The sudden infant death syndrome (SIDS) is the main cause of postneonatal infant death and its cause is still unknown. Neuromodulin/GAP43 is a marker of synaptic plasticity and is critical for normal development of the serotonergic innervation. Neuromodulin/GAP43 is a major cortical cytoskeleton-associated and calmodulin binding protein that is widely and abundantly expressed during development, maintained in selected brain structures in the adult, and reinduced during nerve regeneration. CAP23 and GAP43 are functionally related intrinsic determinants of anatomical plasticity. These proteins function by locally promoting subplasmalemmal actin cytoskeleton accumulation.

References

Frey,D. et al., 2000, J Cell Biol. 149 (7):1443-54. Caprini,M. et al., 2003, EMBO J. 22 (12):3004-14. Sawaguchi,T. et al., 2003, Early Hum Dev. 75 Suppl :S139-46. Kowara,R. et al., 2007, Biochem Biophys Res Commun 363 (1):190-3.

NCBI and Uniprot Product Information

• NCBI GI #: 194248057
• NCBI GeneID: 2596
• NCBI Accession #: NP_001123536.1
• NCBI GenBank Nucleotide #: NM_001130064.1
• UniProt Accession #: P17677; A8K0Y4
• Molecular Weight: 24,803 Da
• NCBI Official Full Name: neuromodulin isoform 1
• NCBI Official Synonym Full Names: growth associated protein 43
• NCBI Official Symbol: GAP43
• NCBI Official Synonym Symbols: B-50; PP46
• NCBI Protein Information: neuromodulin; protein F1; neural phosphoprotein B-50; growth-associated protein 43; axonal membrane protein GAP-43; calmodulin-binding protein P-57; nerve growth-related peptide GAP43; neuron growth-associated protein 43
• UniProt Protein Name: Neuromodulin
• Protein Family: Neuromodulin
• UniProt Gene Name: GAP43
• UniProt Entry Name: NEUM_HUMAN

NCBI Description

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GAP43: This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Belongs to the neuromodulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: filopodium membrane; axon; cytoplasm; postsynaptic density; plasma membrane; cell junction

Molecular Function: calmodulin binding; protein binding

Biological Process: cell fate commitment; glial cell differentiation; tissue regeneration; regulation of growth; axon choice point recognition; protein kinase C activation; response to wounding; regulation of filopodium formation