Rabbit DARPP-32 Polyclonal Antibody | anti-DARPP-32 antibody

RABBIT ANTI DARPP-32 (pThr34)

Cat. Num. AAA221787

• Gene Names: Ppp1r1b; Darpp32; Darpp-32
• Reactivity: Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Monkey (Expected from Sequence), Mouse (Expected from Sequence)
• Applications: Western Blot
• Synonyms: DARPP-32; Polyclonal Antibody; RABBIT ANTI DARPP-32 (pThr34); anti-DARPP-32 antibody

• Host: Rabbit
• Reactivity: Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Monkey (Expected from Sequence), Mouse (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Sequence Length: 205

• Applicable Applications for anti-DARPP-32 antibody: Western Blot (WB)*
• Application Notes: Western Blot: This item detects a band of approximately 32kDa in rat caudate lysates.; Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, threonine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
• Perservative Stabilisers: 0.09% Sodium Azide; 0.1% Bovine Serum Albumin; 50% Glycerol
• Buffer Solution: 10mM HEPES pH7.5
• Antiserum Preparation: Antisera to phosphorylated rat DARPP-32 were raised by repeated immunisations of rabbits with highly purified antigen.
• Target Species: Rat
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of despatch.

Related Product Information for anti-DARPP-32 antibody

Rabbit anti Rat DARP-32 (pThr34) antibody recognizes rat DARPP-32, when phosphorylated at threonine 34. DARPP-32 is also known as Protein phosphatase 1 regulatory subunit 1B or Dopamine- and cAMP-regulated neuronal phosphoprotein. DARPP-32 is a 205 amino acid ~32 kDa member of the protein phosphatase inhibitor 1 family. DARPP-32 is principally expressed in striatal medium spiny neurons, and plays a critical role in the regulation of dopaminergic neurotransmission. DARPP-32 can act either as a phosphatase inhibitor or as a kinase inhibitor, depending on its relative state of phosphorylation. Phosphorylation at threonine 34 converts DARPP-32 into an inhibitor of protein phosphatase-1 (PP-1) whilst phosphorylation at threonine 75 switches the protein to an inhibitor of protein kinase A (PKA). G-protein coupled receptor 6 deficiency in a mouse model of Parkinsons disease leads to an increase in DARPP-32 (pThr34) in striatopalladial neurons with a concommitent increase in locomotor activity and reduced abnormal movements in the mouse dyskinesia model of Parkinson's disease, thus suggesting treatment other than dopamine replacement for the condition (Oekl et al. 2014).

NCBI and Uniprot Product Information

• NCBI GI #: 61889054
• NCBI GeneID: 360616
• NCBI Accession #: NP_612530.1
• NCBI GenBank Nucleotide #: NM_138521.1
• UniProt Accession #: Q6J4I0
• Molecular Weight: 22,913 Da
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 1B
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory (inhibitor) subunit 1B
• NCBI Official Symbol: Ppp1r1b
• NCBI Official Synonym Symbols: Darpp32; Darpp-32
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 1B; dopamine- and cAMP-regulated neuronal phosphoprotein; dopamine- and cAMP-regulated phosphoprotein DARPP-32; neuronal phosphoprotein DARPP-32
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 1B
• UniProt Gene Name: Ppp1r1b
• UniProt Entry Name: PPR1B_RAT

NCBI Description

potent inhibitor of protein phosphatase 1 (PP1); may play a role in maintaining the levels of excitability of NMDA receptors [RGD, Feb 2006]

Uniprot Description

DARPP-32: a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1 inhibitor. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32. Two alternatively-spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Cellular Component: cell soma; cytoplasm; cytosol; nucleus

Molecular Function: protein phosphatase inhibitor activity; D4 dopamine receptor binding; D1 dopamine receptor binding; type 1 serine/threonine specific protein phosphatase inhibitor activity; D3 dopamine receptor binding; D2 dopamine receptor binding; D5 dopamine receptor binding

Biological Process: negative regulation of female receptivity; transcription, DNA-dependent; response to amphetamine; negative regulation of protein kinase activity; visual learning