Goat STAT3 Polyclonal Antibody | anti-STAT3 antibody

GOAT ANTI STAT3 (C-TERMINAL)

Cat. Num. AAA221369

• Gene Names: STAT3; APRF; HIES; ADMIO
• Reactivity: Mouse, Rat (Expected from Sequence)
• Applications: Immunohistochemistry, ELISA, Western Blot
• Synonyms: STAT3; Polyclonal Antibody; GOAT ANTI STAT3 (C-TERMINAL); anti-STAT3 antibody

• Host: Goat
• Reactivity: Mouse, Rat (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5 mg/ml (varies by lot)
• Sequence Length: 722

• Applicable Applications for anti-STAT3 antibody: Immunohistology Frozen*, ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: Goat anti STAT3 antibody detects a band of approximately 80-85kDa in A431 cell lysates. A minimum incubation time of 1 hour is recommended with this antibody. ; Immunohistology - Frozen: Maximum Dilution: 10ug/ml; Application Note: Overnight staining is recommended for this antibody; ELISA: Maximum Dilution: 1/32,000; Western Blotting: Minimum Dilution: 0; Maximum Dilution: 5ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide; 0.5% Bovine Serum Albumin
• Histology Positive Control Tissue: Mouse Thymus.; Buffer Solution
• Antiserum Preparation: Antisera to human STAT3 (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-STAT3 antibody

Goat anti Mouse STAT3 antibody recognizes an epitope within the C-terminal (CT) region of signal transducer and activator of transcription 3 (STAT3), an 80-85 kDa member of the STAT protein family, which plays an important role in cell growth, survival differentiation and transformation, transducing signals from cytokine receptors to the nucleus. STAT3 is phosphorylated in response to cytokines and growth factors including interleukin-5, interleukin-6, epidermal growth factor and leukaemia inhibitory factor. It is expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

NCBI and Uniprot Product Information

• NCBI GI #: 21618338
• NCBI GeneID: 6774
• NCBI Accession #: NP_003141.2
• NCBI GenBank Nucleotide #: NM_003150.3
• UniProt Accession #: P40763; O14916; Q9BW54; A8K7B8; K7ENL3
• Molecular Weight: 83,126 Da
• NCBI Official Full Name: signal transducer and activator of transcription 3 isoform 2
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 3 (acute-phase response factor)
• NCBI Official Symbol: STAT3
• NCBI Official Synonym Symbols: APRF; HIES; ADMIO
• NCBI Protein Information: signal transducer and activator of transcription 3; DNA-binding protein APRF; acute-phase response factor
• UniProt Protein Name: Signal transducer and activator of transcription 3
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT3
• UniProt Synonym Gene Names: APRF
• UniProt Entry Name: STAT3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Transcription factor; DNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; cytoplasm; mitochondrial inner membrane; plasma membrane; nucleus; cytosol

Molecular Function: protein dimerization activity; protein binding; signal transducer activity; ligand-dependent nuclear receptor activity; DNA binding; sequence-specific DNA binding; transcription factor activity; protein phosphatase binding; transcription factor binding; protein kinase binding; CCR5 chemokine receptor binding; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; viral reproduction; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; radial glial cell differentiation; thermoregulation; negative regulation of transcription from RNA polymerase II promoter; signal transduction; glucose homeostasis; response to estradiol stimulus; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; astrocyte differentiation; protein import into nucleus; acute-phase response; negative regulation of glycolysis; positive regulation of Notch signaling pathway; response to drug; nervous system development; intracellular receptor-mediated signaling pathway; eating behavior; cytokine and chemokine mediated signaling pathway; regulation of multicellular organism growth; JAK-STAT cascade; cellular response to hormone stimulus; regulation of transcription from RNA polymerase II promoter; cell proliferation; response to ethanol; sexual reproduction; positive regulation of transcription from RNA polymerase II promoter; eye photoreceptor cell differentiation; cell motility; phosphorylation

Disease: Hyper-ige Recurrent Infection Syndrome, Autosomal Dominant; Autoimmune Disease, Multisystem, Infantile-onset