Goat IL-6 Polyclonal Antibody | anti-Il6 antibody

GOAT ANTI RAT INTERLEUKIN-6

Cat. Num. AAA221297

• Gene Names: Il6; ILg6; Ifnb2
• Applications: ELISA, Functional Assay, Western Blot
• Synonyms: IL-6; Polyclonal Antibody; GOAT ANTI RAT INTERLEUKIN-6; anti-Il6 antibody

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This product, Goat anti Rat IL-6 antibody (purified), recognizes rat interleukin 6.IL-6 is a pleiotropic cytokine involved in the regulation of immune and inflammatory responses.
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 0.1 mg/ml after reconstitution (varies by lot)
• Sequence Length: 211

• Applicable Applications for anti-Il6 antibody: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Anti rat IL-6: purified (This item) may be used in an indirect ELISA with recombinant rat IL-6 as the standard.; ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml; Functional Assays: Minimum Dilution: 2ng/ml; Maximum Dilution: 5ng/ml; Western Blotting: Minimum Dilution: 0.1ug/ml; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 1.0 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide.
• Immunogen: Recombinant Rat IL-6 (PRP21); Buffer Solution
• Antiserum Preparation: Antisera to rat IL-6 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Rat
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Related Product Information for anti-Il6 antibody

This product, Goat anti Rat IL-6 antibody (purified), recognizes rat interleukin 6. IL-6 is a pleiotropic cytokine involved in the regulation of immune and inflammatory responses.

NCBI and Uniprot Product Information

• NCBI GI #: 7549769
• NCBI GeneID: 24498
• NCBI Accession #: NP_036721.1
• NCBI GenBank Nucleotide #: NM_012589.2
• UniProt Accession #: P20607
• Molecular Weight: 24,357 Da
• NCBI Official Full Name: interleukin-6
• NCBI Official Synonym Full Names: interleukin 6
• NCBI Official Symbol: Il6
• NCBI Official Synonym Symbols: ILg6; Ifnb2
• NCBI Protein Information: interleukin-6; IL-6; Interleukin 6 (interferon, beta 2)
• UniProt Protein Name: Interleukin-6
• Protein Family: Interleukin
• UniProt Gene Name: Il6
• UniProt Synonym Gene Names: Il-6; IL-6
• UniProt Entry Name: IL6_RAT

NCBI Description

a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]

Uniprot Description

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation ().