Rabbit G PROTEIN ALPHA 0 Polyclonal Antibody | anti-Gnao1 antibody

RABBIT ANTI MOUSE G PROTEIN ALPHA 0

Cat. Num. AAA220900

• Gene Names: Gnao1; Gnao; alphaO; Galphao; AW050213
• Reactivity: Reacts with: Drosophila; N.B. Antibody reactivity and working conditions may vary between species.
• Applications: ELISA, Western Blot
• Synonyms: G PROTEIN ALPHA 0; Polyclonal Antibody; RABBIT ANTI MOUSE G PROTEIN ALPHA 0; anti-Gnao1 antibody

• Host: Rabbit
• Reactivity: Reacts with: Drosophila; N.B. Antibody reactivity and working conditions may vary between species.
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Rabbit anti Mouse G Protein alpha 0 antibody recognizes the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40kDa forming a heterotrimeric protein complex with beta and Gamma subunits. Two isoforms of the ? subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 5.0 mg/ml (varies by lot)
• Sequence: {C-}A-N-N-L-R-G-C-G-L-Y
• Sequence Length: 354

• Applicable Applications for anti-Gnao1 antibody: ELISA (EIA), Western Blot (WB)*
• Application Notes: ELISA: Minimum Dilution: 1/500; Maximum Dilution: 1/1000;; Western Blotting: Application Note: This antibody detects band at 39 kDa when used under reducing conditions against rat brain.
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Preparation: Purified IgG prepared by affinity chromatography on Protein G
• Immunogen: Synthetic peptide corresponding to the amino acids 345 - 354 of the native isoform 1 molecule conjugated to Keyhole Limpet Haemocyanin.
• Buffer Solution: Phosphate buffered saline
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Synthetic G Protein Alpha 0 detected with Rabbit anti Mouse G Protein Alpha 0 (MBS220900))

Related Product Information for anti-Gnao1 antibody

Rabbit anti Mouse G Protein alpha 0 antibody recognizes the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40kDa forming a heterotrimeric protein complex with beta and gamma subunits. Two isoforms of the alpha subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2. Rabbit anti Mouse G Protein alpha 0 antibody has been used successfully for the detection of G(o) in Drosophila by western blotting (Guo et al. 2011).

NCBI and Uniprot Product Information

• NCBI GI #: 164607137
• NCBI GeneID: 14681
• NCBI Accession #: NP_001106855.1
• NCBI GenBank Nucleotide #: NM_001113384.1
• UniProt Accession #: P18872; P18873
• Molecular Weight: 40,036 Da
• NCBI Official Full Name: guanine nucleotide-binding protein G(o) subunit alpha isoform B
• NCBI Official Synonym Full Names: guanine nucleotide binding protein, alpha O
• NCBI Official Symbol: Gnao1
• NCBI Official Synonym Symbols: Gnao; alphaO; Galphao; AW050213
• NCBI Protein Information: guanine nucleotide-binding protein G(o) subunit alpha; guanine nucleotide-binding protein G(o) subunit alpha; GTP-binding protein alpha-o subunit; Go alpha
• UniProt Protein Name: Guanine nucleotide-binding protein G(o) subunit alpha
• UniProt Gene Name: Gnao1
• UniProt Synonym Gene Names: Gna0; Gnao
• UniProt Entry Name: GNAO_MOUSE

Uniprot Description

G-alpha(o): Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear. Stimulated by RGS14. Interacts with RGS14. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Belongs to the G-alpha family. G(i/o/t/z) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein; G protein, heterotrimeric alpha G((i/o/t/z)); G protein, heterotrimeric

Cellular Component: neuron projection; protein complex; membrane; dendrite; plasma membrane; heterotrimeric G-protein complex; intracellular; myelin sheath

Molecular Function: GTPase activity; corticotropin-releasing hormone receptor 1 binding; GTP binding; guanyl nucleotide binding; metal ion binding; nucleotide binding; GTPase activating protein binding; signal transducer activity; protein binding; mu-type opioid receptor binding; G-protein beta/gamma-subunit binding; protein complex binding; metabotropic serotonin receptor binding

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; negative regulation of calcium ion transport; metabolic process; dopamine receptor signaling pathway; locomotory behavior; signal transduction; response to organic nitrogen; cellular process; regulation of heart contraction; positive regulation of GTPase activity; aging