Rabbit MIP-1 ALPHA Polyclonal Antibody | anti-MIP-1 ALPHA antibody
RABBIT ANTI RAT MIP-1 ALPHA:Biotin
Gene Names
Ccl3; Scya3; MIP-1a
Applications
ELISA, Western Blot
Synonyms
MIP-1 ALPHA; Polyclonal Antibody; RABBIT ANTI RAT MIP-1 ALPHA:Biotin; anti-MIP-1 ALPHA antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Form/Format
Biotin
Purified IgG conjugated to Biotin - lyophilised
Purified IgG conjugated to Biotin - lyophilised
Concentration
IgG concentration 50 ug/ml (varies by lot)
Sequence Length
92
Applicable Applications for anti-MIP-1 ALPHA antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: Minimum Dilution: 0.15ug/ml; Maximum Dilution: 0.30ug/ml;
Western Blotting: Minimum Dilution: 0.1ug/ml; Maximum Dilution: 0.2ug/ml
Western Blotting: Minimum Dilution: 0.1ug/ml; Maximum Dilution: 0.2ug/ml
Reconstitution
Reconstitute with 1.0 ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
Immunogen
Recombinant rat MIP-1 alpha
Buffer Solution
Buffer Solution
Antiserum Preparation
Antisera to rat MIP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Rat
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. The addition of 0. 09% sodium azide is recommended for long term storage.
Shelf Life: 12 months from date of reconstitution.
Shelf Life: 12 months from date of reconstitution.
Related Product Information for anti-MIP-1 ALPHA antibody
Rabbit anti Rat MIP-1 alpha antibody recognizes rat macrophage inflammatory protein-1 alpha (MIP-1 alpha), also known as CCL3. MIP-1 alpha has a molecular weight of approximately 7.8kD, which is produced by activated macrophages. MIP-1 alpha has been shown to be involved in the inflammatory response of blood monocytes and tissue macrophages.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,335 Da
NCBI Official Full Name
C-C motif chemokine 3
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 3
NCBI Official Symbol
Ccl3
NCBI Official Synonym Symbols
Scya3; MIP-1a
NCBI Protein Information
C-C motif chemokine 3; MIP-1-alpha; Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3); macrophage inflammatory protein 1-alpha; small inducible cytokine A3; small-inducible cytokine A3
UniProt Protein Name
C-C motif chemokine 3
UniProt Gene Name
Ccl3
UniProt Synonym Gene Names
Mip1a; Scya3; MIP-1-alpha
UniProt Entry Name
CCL3_RAT
NCBI Description
mediates monocyte and neutrophil chemotaxis; may play a role in the pathogenesis of acute lung injury [RGD, Feb 2006]
Uniprot Description
Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.
Research Articles on MIP-1 ALPHA
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Product Notes
The MIP-1 ALPHA ccl3 (Catalog #AAA220153) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MIP-1 ALPHA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: Minimum Dilution: 0.15ug/ml; Maximum Dilution: 0.30ug/ml; Western Blotting: Minimum Dilution: 0.1ug/ml; Maximum Dilution: 0.2ug/ml. Researchers should empirically determine the suitability of the MIP-1 ALPHA ccl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIP-1 ALPHA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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