Testing Data
(Staining of paraffin embedded human brain with Mouse anti Bovine GFAP following pronase treatment)
Mouse GFAP Monoclonal Antibody | anti-GFAP antibody
MOUSE ANTI BOVINE GFAP
Reactivity
Chicken, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep
Applications
Immunohistochemistry, Immunohistochemistry, immunoassay, Radioimmunoassay, Western Blot
Synonyms
GFAP; Monoclonal Antibody; MOUSE ANTI BOVINE GFAP; anti-GFAP antibody
Host
Mouse
Reactivity
Chicken, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
1B4
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
430
Applicable Applications for anti-GFAP antibody
Immunohistology Frozen, Immunohistology Paraffin*, Radioimmunoassays (RIA), Western Blot (WB)
Application Notes
Immunohistology - Paraffin: Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Preparation
Immunogen
Bovine spinal cord homogenate.
Histology Positive Control Tissue
Histology Positive Control Tissue
Target Species
Bovine
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Staining of paraffin embedded human brain with Mouse anti Bovine GFAP following pronase treatment)
Testing Data
(Staining of paraffin embedded human brain with Mouse anti Bovine GFAP following pronase treatment)
Related Product Information for anti-GFAP antibody
Mouse anti Bovine GFAP antibody, clone 1B4 recognizes glial fibrillary acidic protein, or GFAP, a class III intermediate filament. During development of the central nervous system, GFAP distinguishes astrocytes from other glial cells. It is involved in various cellular functions, such as cell structure and movement, cell communication and the functioning of the blood-brain barrier. It also plays a role in mitosis by adjusting the filament network present in the cell. Defects in GFAP are a cause of Alexander disease, a rare disorder of the central nervous system affecting mostly males. It is a progressive leukoencephalopathy resulting in mental and physical retardation, dementia, seizures and early death. Mouse anti Bovine GFAP antibody, clone 1B4 may be used in conjunction with clones 4A11 and 2E1 for increased sensitivity when used in immunohistology.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,782 Da
NCBI Official Full Name
glial fibrillary acidic protein
NCBI Official Synonym Full Names
glial fibrillary acidic protein
NCBI Official Symbol
Gfap
NCBI Protein Information
glial fibrillary acidic protein; glial fibrillary acidic protein alpha; intermediate filament
UniProt Protein Name
Glial fibrillary acidic protein
Protein Family
UniProt Gene Name
Gfap
UniProt Synonym Gene Names
GFAP
UniProt Entry Name
GFAP_RAT
NCBI Description
intermediate filament protein specific for astrocytes [RGD, Feb 2006]
Uniprot Description
GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined.
Protein type: Cytoskeletal
Cellular Component: cell projection; membrane; cytoplasm; intermediate filament; myelin sheath
Molecular Function: integrin binding; structural constituent of cytoskeleton; structural molecule activity; kinase binding
Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; intermediate filament organization; neurite regeneration; intermediate filament-based process; astrocyte development
Research Articles on GFAP
Similar Products
Product Notes
The GFAP gfap (Catalog #AAA219613) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI BOVINE GFAP reacts with Chicken, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's GFAP can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Immunohistology Paraffin*, Radioimmunoassays (RIA), Western Blot (WB). Immunohistology - Paraffin: Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Researchers should empirically determine the suitability of the GFAP gfap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFAP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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