Mouse GFAP Monoclonal Antibody | anti-GFAP antibody

Anti-GFAP antibody (monoclonal)

Cat. Num. AAA175099

• Reactivity: Human, Pig, Rat
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry
• Purity: Ascites
• Synonyms: GFAP; Monoclonal Antibody; Anti-GFAP antibody (monoclonal); Glial fibrillary acidic protein; glial fibrillary acidic protein; FLJ45472; intermediate filament protein; anti-GFAP antibody

• Host: Mouse
• Reactivity: Human, Pig, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: GA-8
• Purity/Purification: Ascites
• Form/Format: Lyophilized
• Sequence Length: 432

• Applicable Applications for anti-GFAP antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin
• Application Notes: WB: Concentration: 0.5-1ug/ml; Tested Species: Human, Pig, Rat; IHC-P: Concentration: 0.4-1ug/ml; Tested Species: Human, Pig, Rat; Antigen Retrieval: By Heat; IHC-F: Concentration: 0.5-1ug/ml; Tested Species: Human, Pig, Rat; ; Other applications have not been tested. Optimal dilutions should be determined by end user.; ; Antibody can be supported by chemiluminescence kit MBS176445 in WB, supported by MBS176438 in IHC-P and IHC-F.
• Immunogen: GFAP from pig spinal cord.
• Contents: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
• Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti- GFAP antibody, MBS175099, IHC(P)IHC(P): Rat Brain Tissue )

Related Product Information for anti-GFAP antibody

Glial fibrillary acidic protein(GFAP) is an intermediate filament protein of 52Kda. GFAP gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.

NCBI and Uniprot Product Information

• NCBI GI #: 121135
• NCBI GeneID: 2670
• UniProt Accession #: P14136; Q53H98; Q5D055; Q6ZQS3; Q7Z5J6; Q7Z5J7; Q96KS4; Q96P18; Q9UFD0; B2RD44; D3DX59; E9PAX3
• NCBI Official Full Name: Glial fibrillary acidic protein
• NCBI Official Synonym Full Names: glial fibrillary acidic protein
• NCBI Official Symbol: GFAP
• NCBI Protein Information: glial fibrillary acidic protein
• UniProt Protein Name: Glial fibrillary acidic protein
• Protein Family: Glial fibrillary acidic protein
• UniProt Gene Name: GFAP
• UniProt Synonym Gene Names: GFAP
• UniProt Entry Name: GFAP_HUMAN

NCBI Description

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: membrane; cytoplasm; intermediate filament; cytosol

Molecular Function: integrin binding; structural constituent of cytoskeleton; kinase binding

Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; neurite regeneration; intermediate filament organization; astrocyte development

Disease: Alexander Disease