Mouse AQUAPORIN 1 Monoclonal Antibody | anti-Aqp1 antibody
MOUSE ANTI AQUAPORIN 1
Gene Names
Aqp1; CHIP28
Applications
Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA, Western Blot, Immunoprecipitation
Synonyms
AQUAPORIN 1; Monoclonal Antibody; MOUSE ANTI AQUAPORIN 1; anti-Aqp1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
7D11
Specificity
AQUAPORIN 1
This item recognises an epitope within an extracellular region of the water-specific channel aquaporin 1 (also known as AQP1 or CHIP-28)
Aquaporin 1 is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kD in mass.
This item recognises an epitope within an extracellular region of the water-specific channel aquaporin 1 (also known as AQP1 or CHIP-28)
Aquaporin 1 is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kD in mass.
Form/Format
Purified (Purified IgG - liquid)
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
269
Applicable Applications for anti-Aqp1 antibody
Immunohistochemistry-Paraffin (IHC), Immunohistochemistry-Frozen (IHC-Fr), Flow Cytometry (FACS), ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
ELISA: 1/1000 - 1/10000
Western Blotting: 1/1000 - 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
(1) This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: 1/1000 - 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
(1) This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Immunogen
Synthetic peptide corresponding to amino acids 198-208 of rat aquaporin 1.
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with the cells of Sp-2/0 Ag14 mouse myeloma cell line.
Cross Reactivity
Reacts with: Mouse, Human
N.B. Antibody reactivity and working conditions may vary between species.
N.B. Antibody reactivity and working conditions may vary between species.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilizers
0.1% Sodium Azide
0.1% Bovine Serum Albumin
0.1% Bovine Serum Albumin
Preparation and Storage
Store at 4°C or at -20°C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
aquaporin-1
NCBI Official Synonym Full Names
aquaporin 1
NCBI Official Symbol
Aqp1
NCBI Official Synonym Symbols
CHIP28
NCBI Protein Information
aquaporin-1; AQP-1; aquaporin-CHIP; Aquaporin 1 (aquaporin channel forming integral protein 28 (CHIP)); water channel protein for red blood cells and kidney proximal tubule
UniProt Protein Name
Aquaporin-1
UniProt Gene Name
Aqp1
UniProt Synonym Gene Names
Chip28; AQP-1
UniProt Entry Name
AQP1_RAT
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Product Notes
The Aqp1 aqp1 (Catalog #AAA212491) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AQUAPORIN 1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC), Immunohistochemistry-Frozen (IHC-Fr), Flow Cytometry (FACS), ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). ELISA: 1/1000 - 1/10000 Western Blotting: 1/1000 - 1/2000 Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. (1) This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. Researchers should empirically determine the suitability of the Aqp1 aqp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AQUAPORIN 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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