Human Cytochrome C Oxidase Subunit IV Isoform 2 (COX4I2) ELISA Kit | COX4I2 elisa kit

Human Cytochrome C Oxidase Subunit IV Isoform 2 (COX4I2) ELISA Kit

Cat. Num. AAA2122238

• Gene Names: COX4I2; COX4; COX4B; COX4-2; COX4L2; COXIV-2; dJ857M17.2
• Reactivity: Human
• Synonyms: Cytochrome C Oxidase Subunit IV Isoform 2 (COX4I2); Human Cytochrome C Oxidase Subunit IV Isoform 2 (COX4I2) ELISA Kit; COX4; COX4-2; COX4B; COX4L2; COXIV-2; Cytochrome C Oxidase Subunit IV-Like 2; Cytochrome c oxidase subunit 4 isoform 2; mitochondrial; COX4I2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cytochrome C Oxidase Subunit IV Isoform 2 (COX4I2).; No significant cross-reactivity or interference between Cytochrome C Oxidase Subunit IV Isoform 2 (COX4I2) and analogues was observed.
• Sequence Length: 171

• Assay Type: Double-antibody Sandwich
• Sample: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Dectection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.059ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome C Oxidase Subunit IV Isoform 2 (COX4I2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome C Oxidase Subunit IV Isoform 2 (COX4I2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COX4I2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome C Oxidase Subunit IV Isoform 2 (COX4I2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome C Oxidase Subunit IV Isoform 2 (COX4I2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome C Oxidase Subunit IV Isoform 2 (COX4I2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome C Oxidase Subunit IV Isoform 2 (COX4I2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for COX4I2 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 17999526
• NCBI GeneID: 84701
• NCBI Accession #: NP_115998.2
• NCBI GenBank Nucleotide #: NP_115998.2
• UniProt Accession #: Q96KJ9
• NCBI Official Full Name: cytochrome c oxidase subunit 4 isoform 2, mitochondrial
• NCBI Official Synonym Full Names: cytochrome c oxidase subunit 4I2
• NCBI Official Symbol: COX4I2
• NCBI Official Synonym Symbols: COX4; COX4B; COX4-2; COX4L2; COXIV-2; dJ857M17.2
• NCBI Protein Information: cytochrome c oxidase subunit 4 isoform 2, mitochondrial
• UniProt Protein Name: Cytochrome c oxidase subunit 4 isoform 2, mitochondrial
• UniProt Gene Name: COX4I2
• UniProt Synonym Gene Names: COX4L2; COX IV-2
• UniProt Entry Name: COX42_HUMAN

NCBI Description

Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes isoform 2 of subunit IV. Isoform 1 of subunit IV is encoded by a different gene, however, the two genes show a similar structural organization. Subunit IV is the largest nuclear encoded subunit which plays a pivotal role in COX regulation. [provided by RefSeq, Jul 2008]

Uniprot Description

COX4I2: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Defects in COX4I2 are the cause of exocrine pancreatic insufficiency dyserythropoietic anemia and calvarial hyperostosis (EPIDACH). Patients present with pancreatic insufficiency, intestinal malabsorption, failure to thrive, and anemia soon after birth. Belongs to the cytochrome c oxidase IV family.

Protein type: Oxidoreductase; Mitochondrial

Chromosomal Location of Human Ortholog: 20q11.21

Cellular Component: mitochondrial respiratory chain complex IV

Molecular Function: cytochrome-c oxidase activity

Biological Process: generation of precursor metabolites and energy; cellular respiration

Disease: Exocrine Pancreatic Insufficiency, Dyserythropoietic Anemia, And Calvarial Hyperostosis