Testing Data
(Published customer image: Oenothein B Primes bovine CD335+ cells to respond to IL-18. (A) Bovine PBMCs (105 cells/well) were depleted of CD335+ cells and treated with 20 ug/ml oenothein B or X-VIVO medium alone for 24 hrs. Cells were then washed and treated with 10 ng/ml rhu IL-18 or medium alone for 18 hrs. After incubation, IFN? levels in the supernatant fluids were measured by ELISA. The data are expressed as mean +/- SEM of three independent experiments comparing depleted PBMCs to un-depleted controls tested concurrently. All samples were tested in duplicate or triplicate. Statistical significance was measured by Two-way ANOVA with Bonferroni post-test. *p)
Mouse CD335 Monoclonal Antibody | anti-CD335 antibody
MOUSE ANTI BOVINE CD335:Low Endotoxin
Purified IgG - liquid
Flow Cytometry: Minimum Dilution: 1/5; Maximum Dilution: 1/200
Fusion Partners
Shelf Life: 18 months from date of despatch.
Testing Data
(Published customer image: Oenothein B Primes bovine CD335+ cells to respond to IL-18. (A) Bovine PBMCs (105 cells/well) were depleted of CD335+ cells and treated with 20 ug/ml oenothein B or X-VIVO medium alone for 24 hrs. Cells were then washed and treated with 10 ng/ml rhu IL-18 or medium alone for 18 hrs. After incubation, IFN? levels in the supernatant fluids were measured by ELISA. The data are expressed as mean +/- SEM of three independent experiments comparing depleted PBMCs to un-depleted controls tested concurrently. All samples were tested in duplicate or triplicate. Statistical significance was measured by Two-way ANOVA with Bonferroni post-test. *p)
Testing Data
(Published customer image: Oenothein B Primes bovine CD335+ cells to respond to IL-18. (A) Bovine PBMCs (105 cells/well) were depleted of CD335+ cells and treated with 20 ug/ml oenothein B or X-VIVO medium alone for 24 hrs. Cells were then washed and treated with 10 ng/ml rhu IL-18 or medium alone for 18 hrs. After incubation, IFN? levels in the supernatant fluids were measured by ELISA. The data are expressed as mean +/- SEM of three independent experiments comparing depleted PBMCs to un-depleted controls tested concurrently. All samples were tested in duplicate or triplicate. Statistical significance was measured by Two-way ANOVA with Bonferroni post-test. *p)
Testing Data
(Published customer image: Effect of anti-CD2 mAbs on bovine NK cell functions. (A) IFN-? response from NK cells caused by cross-linking of CD2. Plastic wells were pre-coated with two different mAbs against bovine CD2, along with NKp46-mAb as positive control and CD8-mAb or medium as negative controls. rbIL-2 activated NK cells were added and incubated for 24 h in the presence or absence of rbIL-12 (0.5 U/ml), and IFN-? content in the supernatants analyzed by an ELISA. Data shown are means +/- SD of two to four individual animals. (B-C) IL-2 activated NK cell killing of the bovine target lines BL3.1 and MDBK in a 4 h 51Cr-release assay, with addition of the indicated mAbs for blocking. CD2mAb I = BAQ95A, CD2 mAb II = MUC2A, CD8 mAb = CACT80C, NKp46 mAb = AKS1. (D) As above, using murine Fc-receptor bearing P815 target cells to obtain a redirected lysis effect. Data shown in (B-D) are from one animal representative of three to five.From: Boysen et al. BMC Immunology 2006 7:10.)
Testing Data
(Published customer image: Effect of anti-CD2 mAbs on bovine NK cell functions. (A) IFN-? response from NK cells caused by cross-linking of CD2. Plastic wells were pre-coated with two different mAbs against bovine CD2, along with NKp46-mAb as positive control and CD8-mAb or medium as negative controls. rbIL-2 activated NK cells were added and incubated for 24 h in the presence or absence of rbIL-12 (0.5 U/ml), and IFN-? content in the supernatants analyzed by an ELISA. Data shown are means +/- SD of two to four individual animals. (B-C) IL-2 activated NK cell killing of the bovine target lines BL3.1 and MDBK in a 4 h 51Cr-release assay, with addition of the indicated mAbs for blocking. CD2mAb I = BAQ95A, CD2 mAb II = MUC2A, CD8 mAb = CACT80C, NKp46 mAb = AKS1. (D) As above, using murine Fc-receptor bearing P815 target cells to obtain a redirected lysis effect. Data shown in (B-D) are from one animal representative of three to five.From: Boysen et al. BMC Immunology 2006 7:10.)
Testing Data
(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD335 (MBS224513) followed by Goat anti Mouse IgG (H/L):FITC (MBS235142). A small positive peak representing NK cells can be identified)
Testing Data
(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD335 (MBS224513) followed by Goat anti Mouse IgG (H/L):FITC (MBS235142). A small positive peak representing NK cells can be identified)
Testing Data
(Published customer image: Oenothein B induces IL-2Ra or CD69 on bovine and human NK cells. (A) Bovine PBMCs (105 cells/well) were treated with 20 ug/ml oenothein B in X-VIVO medium for 24 hrs, and IL-2Ra expression on NK cells was measured by multi-color flow cytometry. Representative examples of two-color flow cytometry plots comparing IL-2Ra staining on oenothein B-treated and untreated bovine NK cells (CD335+) from each animal are shown. (B) Human PBMCs (105 cells/well) were treated with 40 ug/ml oenothein B in cRPMI medium for 48 hrs. CD69 expression on NK cells was then measured by flow cytometry. Representative examples of two-color flow cytometry plots comparing CD69 staining on oenothein B-treated and untreated human NK cells from each donor are shown.From: Ramstead AG, Schepetkin IA, Quinn MT, Jutila MA (2012) Oenothein B, a Cyclic Dimeric Ellagitannin Isolated from Epilobium angustifolium, Enhances IFN? Production by Lymphocytes. PLoS ONE 7(11): e50546.)
Testing Data
(Published customer image: Oenothein B induces IL-2Ra or CD69 on bovine and human NK cells. (A) Bovine PBMCs (105 cells/well) were treated with 20 ug/ml oenothein B in X-VIVO medium for 24 hrs, and IL-2Ra expression on NK cells was measured by multi-color flow cytometry. Representative examples of two-color flow cytometry plots comparing IL-2Ra staining on oenothein B-treated and untreated bovine NK cells (CD335+) from each animal are shown. (B) Human PBMCs (105 cells/well) were treated with 40 ug/ml oenothein B in cRPMI medium for 48 hrs. CD69 expression on NK cells was then measured by flow cytometry. Representative examples of two-color flow cytometry plots comparing CD69 staining on oenothein B-treated and untreated human NK cells from each donor are shown.From: Ramstead AG, Schepetkin IA, Quinn MT, Jutila MA (2012) Oenothein B, a Cyclic Dimeric Ellagitannin Isolated from Epilobium angustifolium, Enhances IFN? Production by Lymphocytes. PLoS ONE 7(11): e50546.)
Testing Data
(Published customer image: CD2 distribution on bovine NK cells in peripheral blood and during cell culture. (A) CD2 expression by PBMCs from healthy cattle, and in NKp46-selected cells cultured for the indicated time in the presence of 100U/ml rbIL-2. Results were obtained by two-colour flow cytometry, gating for viable lymphocytes. Data shown are from one animal representative of 19. (B) Monitoring of cell divisions of CD2- and CD2+ NK cell populations during three and six days of rbIL-2 (100U/ml) stimulation. NKp46-selected cells were labelled with CFSE the day after isolation and CFSE intensity (filled histograms) measured in flow cytometry on the indicated days, gating for CD2- or CD2+ viable cells. Solid line open histograms show non-dividing NK cells, cultured in the presence of 1U/ml rbIL-2, while broken line open histograms show unlabeled stimulated NK cells. Data shown are from one animal representative of five. (C) Stability of CD2 expression in NK cell subsets cultured separately. NK cells separated with immunomagnetic beads for absence or presence of CD2 were cultured separately for eleven days in the presence of IL-2, monitoring CD2 expression at three different time points (filled histograms). Day 1 corresponds to the day of separation, 24 h after primary NK cell isolation. Open histograms show secondary mAb controls. Data shown are from one animal representative of eight.From: Boysen et al. BMC Immunology 2006 7:10.)
Testing Data
(Published customer image: CD2 distribution on bovine NK cells in peripheral blood and during cell culture. (A) CD2 expression by PBMCs from healthy cattle, and in NKp46-selected cells cultured for the indicated time in the presence of 100U/ml rbIL-2. Results were obtained by two-colour flow cytometry, gating for viable lymphocytes. Data shown are from one animal representative of 19. (B) Monitoring of cell divisions of CD2- and CD2+ NK cell populations during three and six days of rbIL-2 (100U/ml) stimulation. NKp46-selected cells were labelled with CFSE the day after isolation and CFSE intensity (filled histograms) measured in flow cytometry on the indicated days, gating for CD2- or CD2+ viable cells. Solid line open histograms show non-dividing NK cells, cultured in the presence of 1U/ml rbIL-2, while broken line open histograms show unlabeled stimulated NK cells. Data shown are from one animal representative of five. (C) Stability of CD2 expression in NK cell subsets cultured separately. NK cells separated with immunomagnetic beads for absence or presence of CD2 were cultured separately for eleven days in the presence of IL-2, monitoring CD2 expression at three different time points (filled histograms). Day 1 corresponds to the day of separation, 24 h after primary NK cell isolation. Open histograms show secondary mAb controls. Data shown are from one animal representative of eight.From: Boysen et al. BMC Immunology 2006 7:10.)
NCBI and Uniprot Product Information
Uniprot Description
Cytotoxicity activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
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Product Notes
The CD335 ncr1 (Catalog #AAA211240) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD335 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Functional Assays (FN), Immunofluorescence (IF), Western Blot (WB). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/5; Maximum Dilution: 1/200. Researchers should empirically determine the suitability of the CD335 ncr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD335, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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