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Western Blot (WB) (Western Blot: Sample: Recombinant ALDOA, Rat.)

Rabbit anti-Rat Aldolase A (ALDOA) Polyclonal Antibody | anti-ALDOA antibody

Polyclonal Antibody to Aldolase A, Fructose Bisphosphate (ALDOA)

Gene Names
Aldoa; Aldo1; RNALDOG5
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Aldolase A (ALDOA); Polyclonal Antibody; Polyclonal Antibody to Aldolase A; Fructose Bisphosphate (ALDOA); FBA; ALDO-A; Fructose 1; 6 Bisphosphate Aldolase; Lung cancer antigen NY-LU-1; Muscle-type aldolase; anti-ALDOA antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ALDOA. It has been selected for its ability to recognize ALDOA in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
364
Applicable Applications for anti-ALDOA antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western Blot: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Immunogen
Recombinant Aldolase A, Fructose Bisphosphate (ALDOA)
Cross Reactivity
Rat
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant ALDOA, Rat.)

Western Blot (WB) (Western Blot: Sample: Recombinant ALDOA, Rat.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,352 Da
NCBI Official Full Name
fructose-bisphosphate aldolase A
NCBI Official Synonym Full Names
aldolase, fructose-bisphosphate A
NCBI Official Symbol
Aldoa
NCBI Official Synonym Symbols
Aldo1; RNALDOG5
NCBI Protein Information
fructose-bisphosphate aldolase A
UniProt Protein Name
Fructose-bisphosphate aldolase A
UniProt Gene Name
Aldoa

NCBI Description

member of the aldolase enzyme family [RGD, Feb 2006]

Uniprot Description

ALDOA: a glycolytic enzyme that catalyzes D-fructose 1,6-bisphosphate -> glycerone phosphate + D-glyceraldehyde 3-phosphate. Three forms of aldolase are found in vertebrates - aldolase A in muscle, aldolase B in liver and aldolase C in brain.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 4.1.2.13; Lyase; Mitochondrial

Chromosomal Location of Human Ortholog: 1q36

Cellular Component: actin cytoskeleton; cytoplasm; cytosol; extracellular space; I band; M band; membrane; mitochondrion; myelin sheath; nuclear heterochromatin; nucleus; protein complex; Z disc

Molecular Function: cadherin binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; identical protein binding; protease binding; protein binding; RNA binding

Biological Process: ATP biosynthetic process; fructose 1,6-bisphosphate metabolic process; fructose metabolic process; glycolytic process; muscle maintenance; protein homotetramerization; regulation of cell shape; response to estrogen; response to heat; response to hypoxia; response to lipopolysaccharide; response to nicotine; striated muscle contraction

Research Articles on ALDOA

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Product Notes

The ALDOA aldoa (Catalog #AAA2090520) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Aldolase A, Fructose Bisphosphate (ALDOA) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Aldolase A (ALDOA) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL. Researchers should empirically determine the suitability of the ALDOA aldoa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aldolase A (ALDOA), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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