Rabbit anti-Mouse Fatty Acid Binding Protein 4, Adipocyte (FABP4) Polyclonal Antibody | anti-FABP4 antibody

Fatty Acid Binding Protein 4, Adipocyte (FABP4) Polyclonal Antibody

Cat. Num. AAA2090425

• Gene Names: Fabp4; Ap2; P15; ALBP; Lbpl; AFABP; 422/aP2; ALBP/Ap2
• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Fatty Acid Binding Protein 4; Adipocyte (FABP4); Polyclonal Antibody; Adipocyte (FABP4) Polyclonal Antibody; Polyclonal Antibody to Fatty Acid Binding Protein 4; A-FABP; AFABP; AP2; Adipocyte Protein 2; Adipocyte-type fatty acid-binding protein; anti-FABP4 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against FABP4. It has been selected for its ability to recognize FABP4 in immunohistochemical staining and western blotting.
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Liquid
• Concentration: 500ug/mL (varies by lot)

• Applicable Applications for anti-FABP4 antibody: Western Blot (WB), IHC, Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: Western Blot: 0.5-2ug/ml; Immunocytochemistry in formalin fixed cells: 5-20ug/ml; Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml; Immunohistochemistry in paraffin section: 5-20ug/ml; ELISA: 0.05-2ug/ml
• Organism Species: Mouse
• Immunogen: Eukaryotic Fatty Acid Binding Protein 4, Adipocyte (FABP4)
• Cross Reactivity: Mouse, Rat
• Buffer Formulation: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Mouse Heart Tissue; Lane2: Mouse Liver Tissue; Lane3: Mouse Brain Tissue; Lane4: Rat Heart Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant FABP4, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Intestine Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Spleen Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Brain Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 14149635
• NCBI GeneID: 11770
• NCBI Accession #: NP_077717.1
• NCBI GenBank Nucleotide #: NM_024406.3
• UniProt Accession #: P04117
• Molecular Weight: 14,650 Da
• NCBI Official Full Name: fatty acid-binding protein, adipocyte
• NCBI Official Synonym Full Names: fatty acid binding protein 4, adipocyte
• NCBI Official Symbol: Fabp4
• NCBI Official Synonym Symbols: Ap2; P15; ALBP; Lbpl; AFABP; 422/aP2; ALBP/Ap2
• NCBI Protein Information: fatty acid-binding protein, adipocyte
• UniProt Protein Name: Fatty acid-binding protein, adipocyte
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: Fabp4
• UniProt Synonym Gene Names: Ap2; ALBP; A-FABP; AFABP

Uniprot Description

FABP4: a lipid transport protein that is the predominant fatty acid binding protein found in adipocytes. Also expressed in macrophages. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. Homodimer. Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Interacts with PPARG. Monomer. FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes. Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE. Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders. Belongs to the calycin superfamily,fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 3 A1|3 2.56 cM

Cellular Component: cytoplasm; cytosol; extracellular exosome; nucleus

Molecular Function: fatty acid binding; lipid binding; transporter activity

Biological Process: brown fat cell differentiation; cellular response to lithium ion; cellular response to tumor necrosis factor; cholesterol homeostasis; cytokine production; fatty acid metabolic process; negative regulation of protein kinase activity; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of inflammatory response; regulation of inflammatory response; transport; white fat cell differentiation