Mouse Vascular Endothelial Growth Factor D (VEGFD) ELISA Kit | VEGFD elisa kit

Mouse Vascular Endothelial Growth Factor D (VEGFD) Mini Samples ELISA Kit

Cat. Num. AAA2089511

• Gene Names: VEGFD; FIGF; VEGF-D
• Reactivity: Mouse
• Synonyms: Vascular Endothelial Growth Factor D (VEGFD); Mouse Vascular Endothelial Growth Factor D (VEGFD) Mini Samples ELISA Kit; FIGF; VEGF-D; C-Fos Induced Growth Factor; VEGFD elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Vascular Endothelial Growth Factor D (VEGFD).; No significant cross-reactivity or interference between Mini Samples Vascular Endothelial Growth Factor D (VEGFD) and analogues was observed.
• Sequence Length: 354

• Assay Type: Double-antibody Sandwich
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Vascular Endothelial Growth Factor D (VEGFD) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Vascular Endothelial Growth Factor D (VEGFD) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Related Product Information for VEGFD elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Vascular Endothelial Growth Factor D (VEGFD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Vascular Endothelial Growth Factor D (VEGFD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Vascular Endothelial Growth Factor D (VEGFD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Vascular Endothelial Growth Factor D (VEGFD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for VEGFD elisa kit

Cytokine; Tumor immunity; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 4758378
• NCBI GeneID: 2277
• NCBI Accession #: NP_004460.1
• NCBI GenBank Nucleotide #: NM_004469.4
• UniProt Accession #: O43915; B2R7Z3
• Molecular Weight: 40,444 Da
• NCBI Official Full Name: vascular endothelial growth factor D preproprotein
• NCBI Official Synonym Full Names: vascular endothelial growth factor D
• NCBI Official Symbol: VEGFD
• NCBI Official Synonym Symbols: FIGF; VEGF-D
• NCBI Protein Information: vascular endothelial growth factor D
• UniProt Protein Name: Vascular endothelial growth factor D
• Protein Family: Vascular endothelial growth factor
• UniProt Gene Name: VEGFD
• UniProt Synonym Gene Names: VEGF-D; FIGF

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C. Read-through transcription has been observed between this locus and the upstream PIR (GeneID 8544) locus. [provided by RefSeq, Feb 2011]

Uniprot Description

VEGFD: Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. Belongs to the PDGF/VEGF growth factor family.

Protein type: Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xp22.2

Cellular Component: extracellular region

Molecular Function: chemoattractant activity; growth factor activity; platelet-derived growth factor receptor binding; protein binding; vascular endothelial growth factor receptor 3 binding; vascular endothelial growth factor receptor binding

Biological Process: angiogenesis; induction of positive chemotaxis; platelet degranulation; positive regulation of angiogenesis; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; response to hypoxia; vascular endothelial growth factor receptor signaling pathway