Mouse Vascular Endothelial Growth Factor D (VEGFD) ELISA Kit | VEGFD elisa kit

Mouse Vascular Endothelial Growth Factor D (VEGFD) ELISA Kit

Cat. Num. AAA2021231

• Gene Names: FIGF; VEGFD; VEGF-D
• Reactivity: Mouse
• Synonyms: Vascular Endothelial Growth Factor D (VEGFD); Mouse Vascular Endothelial Growth Factor D (VEGFD) ELISA Kit; FIGF; VEGF-D; C-Fos Induced Growth Factor; VEGFD elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of VEGFD. No significant cross-reactivity or interference between VEGFD and analogues was observed.
• Sequence Length: 354

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: < 5.7pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VEGFD were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VEGFD were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VEGFD elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of VEGFD in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to VEGFD. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to VEGFD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VEGFD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VEGFD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for VEGFD elisa kit

Cytokine; Tumor immunity; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 4758378
• NCBI GeneID: 2277
• NCBI Accession #: NP_004460.1
• NCBI GenBank Nucleotide #: NM_004469.4
• UniProt Accession #: O43915; B2R7Z3
• Molecular Weight: 40,444 Da
• NCBI Official Full Name: vascular endothelial growth factor D preproprotein
• NCBI Official Synonym Full Names: c-fos induced growth factor (vascular endothelial growth factor D)
• NCBI Official Symbol: FIGF
• NCBI Official Synonym Symbols: VEGFD; VEGF-D
• NCBI Protein Information: vascular endothelial growth factor D
• UniProt Protein Name: Vascular endothelial growth factor D
• Protein Family: Vascular endothelial growth factor
• UniProt Gene Name: FIGF
• UniProt Synonym Gene Names: VEGFD; VEGF-D; FIGF
• UniProt Entry Name: VEGFD_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C. Read-through transcription has been observed between this locus and the upstream PIR (GeneID 8544) locus. [provided by RefSeq, Feb 2011]

Uniprot Description

VEGFD: Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. Belongs to the PDGF/VEGF growth factor family.

Protein type: Secreted; Cytokine; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xp22.31

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: protein homodimerization activity; growth factor activity; platelet-derived growth factor receptor binding; vascular endothelial growth factor receptor 3 binding; vascular endothelial growth factor receptor binding; chemoattractant activity

Biological Process: platelet activation; cell proliferation; platelet degranulation; positive chemotaxis; positive regulation of cell division; positive regulation of cell proliferation; angiogenesis; vascular endothelial growth factor receptor signaling pathway; blood coagulation; cell differentiation; induction of positive chemotaxis