Rat Neuropeptide FF (NPFF) DIY ELISA Kit | NPFF diy elisa kit

Rat Neuropeptide FF (NPFF) ELISA Kit DIY Materials

Cat. Num. AAA2089375

• Gene Names: NPFF; FMRFAL
• Reactivity: Rat
• Synonyms: Neuropeptide FF (NPFF); Rat Neuropeptide FF (NPFF) ELISA Kit DIY Materials; FMRFAL; Neuropeptide FF-Amide Peptide Precursor; Pro-FMRFamide-related neuropeptide FF; NPFF diy elisa kit

• Reactivity: Rat
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Neuropeptide FF (NPFF). No significant cross-reactivity or interference between Neuropeptide FF (NPFF) and analogues was observed.
• Sequence Length: 116

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Competitive Inhibition ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 12.5-200pg/mL
• Sensitivity: 4.7pg/mL
• Reagent Contents: Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 2. (Catalog MBS2089472)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for NPFF diy elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 998429241
• NCBI GeneID: 8620
• NCBI Accession #: NP_001307225.1
• NCBI GenBank Nucleotide #: NM_001320296.1
• UniProt Accession #: O15130; Q3SXL4
• Molecular Weight: 12,991 Da
• NCBI Official Full Name: pro-FMRFamide-related neuropeptide FF isoform 2
• NCBI Official Synonym Full Names: neuropeptide FF-amide peptide precursor
• NCBI Official Symbol: NPFF
• NCBI Official Synonym Symbols: FMRFAL
• NCBI Protein Information: pro-FMRFamide-related neuropeptide FF
• UniProt Protein Name: Pro-FMRFamide-related neuropeptide FF
• Protein Family: Pro-FMRFamide-related neuropeptide
• UniProt Gene Name: NPFF
• UniProt Synonym Gene Names: NPSF; NPFF; NPAF

NCBI Description

This gene encodes a member of the FMRFamide related peptide (FARP) family of neuropeptides. The encoded preproprotein is proteolytically processed to generate multiple amidated peptides. These peptides may play a role in the regulation of heart rate and blood pressure and the modulation of morphine-induced antinociception. Patients with hypertension exhibit decreased expression of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

Uniprot Description

NPFF: Morphine modulating peptides. Have wide-ranging physiologic effects, including the modulation of morphine-induced analgesia, elevation of arterial blood pressure, and increased somatostatin secretion from the pancreas. Neuropeptide FF potentiates and sensitizes ASIC1 and ASIC3 channels. Belongs to the FARP (FMRFamide related peptide) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: dendrite; extracellular region; extracellular space; nerve terminal; perikaryon

Molecular Function: G-protein-coupled receptor binding; neuropeptide hormone activity; receptor binding

Biological Process: regulation of excitatory postsynaptic membrane potential; synaptic transmission