Mouse Fibrinogen Beta (FGb) DIY ELISA Kit | FGb diy elisa kit

Mouse Fibrinogen Beta (FGb) ELISA Kit DIY Materials

Cat. Num. AAA2089046

• Gene Names: FGB; HEL-S-78p
• Reactivity: Mouse
• Synonyms: Fibrinogen Beta (FGb); Mouse Fibrinogen Beta (FGb) ELISA Kit DIY Materials; FG-B; Fibrinopeptide B; FGb diy elisa kit

• Reactivity: Mouse
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Fibrinogen Beta (FGb). No significant cross-reactivity or interference between Fibrinogen Beta (FGb) and analogues was observed.
• Sequence Length: 491

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Plasma.
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.67ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for FGb diy elisa kit

Signal transduction; Infection immunity; Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 70906435
• NCBI GeneID: 2244
• NCBI Accession #: NP_005132.2
• NCBI GenBank Nucleotide #: NM_005141.4
• UniProt Accession #: P02675; Q32Q65; Q3KPF2; A0JLR9; B2R7G3
• Molecular Weight: 55,928 Da
• NCBI Official Full Name: fibrinogen beta chain isoform 1 preproprotein
• NCBI Official Synonym Full Names: fibrinogen beta chain
• NCBI Official Symbol: FGB
• NCBI Official Synonym Symbols: HEL-S-78p
• NCBI Protein Information: fibrinogen beta chain
• UniProt Protein Name: Fibrinogen beta chain
• Protein Family: Fibrinogen
• UniProt Gene Name: FGB

NCBI Description

The protein encoded by this gene is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Uniprot Description

FGB: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. Defects in FGB are a cause of congenital afibrinogenemia (CAFBN). This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding.

Protein type: Adaptor/scaffold; Cell surface; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q31.3

Cellular Component: cell surface; external side of plasma membrane; extracellular region; extracellular space; fibrinogen complex; plasma membrane

Molecular Function: cell adhesion molecule binding; chaperone binding; protein binding; receptor binding; structural molecule activity

Biological Process: blood coagulation; cell-matrix adhesion; cellular protein complex assembly; extracellular matrix organization and biogenesis; fibrinolysis; induction of bacterial agglutination; plasminogen activation; platelet degranulation; positive regulation of exocytosis; positive regulation of heterotypic cell-cell adhesion; positive regulation of protein secretion; positive regulation of vasoconstriction; protein polymerization; response to calcium ion

Disease: Afibrinogenemia, Congenital; Dysfibrinogenemia, Congenital