Rat Fibrinogen Beta (FGb) ELISA Kit | FGb elisa kit

Rat Fibrinogen Beta (FGb) ELISA Kit

Cat. Num. AAA2703029

• Gene Names: FGB; HEL-S-78p
• Reactivity: Rat
• Synonyms: Fibrinogen Beta (FGb); Rat Fibrinogen Beta (FGb) ELISA Kit; FG-B; Fibrinopeptide B; FGb elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibrinogen Beta (FGb).; No significant cross-reactivity or interference between Fibrinogen Beta (FGb) and analogues was observed.
• Sequence Length: 491

• Assay Type: Double-antibody Sandwich
• Samples: Plasma
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.73ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrinogen Beta (FGb) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrinogen Beta (FGb) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGb elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibrinogen Beta (FGb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibrinogen Beta (FGb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibrinogen Beta (FGb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibrinogen Beta (FGb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 70906435
• NCBI GeneID: 2244
• NCBI Accession #: NP_005132.2
• NCBI GenBank Nucleotide #: NM_005141.4
• UniProt Accession #: P02675; Q32Q65; Q3KPF2; A0JLR9; B2R7G3
• Molecular Weight: 55,928 Da
• NCBI Official Full Name: fibrinogen beta chain isoform 1 preproprotein
• NCBI Official Synonym Full Names: fibrinogen beta chain
• NCBI Official Symbol: FGB
• NCBI Official Synonym Symbols: HEL-S-78p
• NCBI Protein Information: fibrinogen beta chain
• UniProt Protein Name: Fibrinogen beta chain
• Protein Family: Fibrinogen
• UniProt Gene Name: FGB

NCBI Description

The protein encoded by this gene is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Uniprot Description

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.