Androsterone (ADT) DIY ELISA Kit | ADT diy elisa kit

General Androsterone (ADT) ELISA Kit DIY Materials

Cat. Num. AAA2088356

• Gene Names: Akr1c2; Akr1c21
• Reactivity: Pan-species (General)
• Synonyms: Androsterone (ADT); General Androsterone (ADT) ELISA Kit DIY Materials; 17-Ketosteroid; 17-KS; 3a-Hydroxy-17-Androstanone; ADT diy elisa kit

• Reactivity: Pan-species (General)
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Androsterone (ADT). No significant cross-reactivity or interference between Androsterone (ADT) and analogues was observed.
• Sequence Length: 318

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Competitive Inhibition ELISA for Antigen Detection
• Samples: Serum, plasma and other biological fluids
• Detection Range: 352-90000pg/mL
• Sensitivity: 144pg/mL
• Reagent Contents: Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 2. (Catalog MBS2089472)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

NCBI and Uniprot Product Information

• NCBI GI #: 81884638
• NCBI GeneID: 291283
• UniProt Accession #: Q6AYQ2
• Molecular Weight: 36,135 Da
• NCBI Official Full Name: Aldo-keto reductase family 1 member C21
• NCBI Official Synonym Full Names: aldo-keto reductase family 1, member C2
• NCBI Official Symbol: Akr1c2
• NCBI Official Synonym Symbols: Akr1c21
• NCBI Protein Information: aldo-keto reductase family 1 member C21
• UniProt Protein Name: Aldo-keto reductase family 1 member C21
• UniProt Gene Name: Akr1c21
• UniProt Synonym Gene Names: 17-alpha-HSD

Uniprot Description

AKR1C2: Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha- DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Has a high bile-binding ability. Defects in AKR1C2 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. Belongs to the aldo/keto reductase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.357; EC 1.3.1.20; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 17q12.2

Molecular Function: 3(or 17)-alpha-hydroxysteroid dehydrogenase activity; aldehyde reductase activity; bile acid binding; carboxylic acid binding; ketosteroid monooxygenase activity; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; phenanthrene 9,10-monooxygenase activity; steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity

Biological Process: digestion; epithelial cell differentiation; G-protein coupled receptor protein signaling pathway; positive regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; progesterone metabolic process; prostaglandin metabolic process; steroid metabolic process