Human Neutrophil Activating Protein 3 (NAP3) DIY ELISA Kit | NAP3 diy elisa kit

Human Neutrophil Activating Protein 3 (NAP3) ELISA Kit DIY Materials

Cat. Num. AAA2088322

• Gene Names: Cxcl1; KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
• Reactivity: Human
• Synonyms: Neutrophil Activating Protein 3 (NAP3); Human Neutrophil Activating Protein 3 (NAP3) ELISA Kit DIY Materials; CXCL1; GRO1; GROa; GRO-A; MGSA-A; NAP3; SCYB1; Chemokine C-X-C-Motif Ligand 1; Melanoma Growth Stimulating Activity Alpha; Fibroblast Secretory Protein; NAP3 diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Neutrophil Activating Protein 3 (NAP3). No significant cross-reactivity or interference between Neutrophil Activating Protein 3 (NAP3) and analogues was observed.
• Sequence Length: 96

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: 6.1pg/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for NAP3 diy elisa kit

Cytokine; Tumor immunity; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 6680109
• NCBI GeneID: 14825
• NCBI Accession #: NP_032202.1
• NCBI GenBank Nucleotide #: NM_008176.3
• UniProt Accession #: P12850
• Molecular Weight: 10,254 Da
• NCBI Official Full Name: growth-regulated alpha protein
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 1
• NCBI Official Symbol: Cxcl1
• NCBI Official Synonym Symbols: KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
• NCBI Protein Information: growth-regulated alpha protein
• UniProt Protein Name: Growth-regulated alpha protein
• UniProt Gene Name: Cxcl1
• UniProt Synonym Gene Names: Gro; Gro1; Mgsa; Scyb1; HSF

NCBI Description

This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung. [provided by RefSeq, Apr 2013]

Uniprot Description

CXCL2: Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5 E1|5 44.78 cM

Cellular Component: extracellular space

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: acute inflammatory response; elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; neutrophil chemotaxis; positive regulation of potassium ion transport; positive regulation of superoxide release; response to lipopolysaccharide; response to molecule of bacterial origin