TDP1 recombinant protein

TDP1, 1-608aa, Human, His-tag, Baculovirus

Cat. Num. AAA206643

• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: TDP1; 1-608aa; Human; His-tag; Baculovirus; tyrosyl-DNA phosphodiesterase 1 isoform a; Tyrosyl-DNA phosphodiesterase 1; Tyr-DNA phosphodiesterase 1; TDP1 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPMSQEGDY GRWTISSSDE SEEEKPKPDK PSTSSLLCAR QGAANEPRYT CSEAQKAAHK RKISPVKFSN TDSVLPPKRQ KSGSQEDLGW CLSSSDDELQ PEMPQKQAEK VVIKKEKDIS APNDGTAQRT ENHGAPACHR LKEEEDEYET SGEGQDIWDM LDKGNPFQFY LTRVSGVKPK YNSGALHIKD ILSPLFGTLV SSAQFNYCFD VDWLVKQYPP EFRKKPILLV HGDKREAKAH LHAQAKPYEN ISLCQAKLDI AFGTHHTKMM LLLYEEGLRV VIHTSNLIHA DWHQKTQGIW LSPLYPRIAD GTHKSGESPT HFKADLISYL MAYNAPSLKE WIDVIHKHDL SETNVYLIGS TPGRFQGSQK DNWGHFRLKK LLKDHASSMP NAESWPVVGQ FSSVGSLGAD ESKWLCSEFK ESMLTLGKES KTPGKSSVPL YLIYPSVENV RTSLEGYPAG GSLPYSIQTA EKQNWLHSYF HKWSAETSGR SNAMPHIKTY MRPSPDFSKI AWFLVTSANL SKAAWGALEK NGTQLMIRSY ELGVLFLPSA FGLDSFKVKQ KFFAGSQEPM ATFPVPYDLP PELYGSKDRP WIWNIPYVKA PDTHGNMWVP SHHHHHH
• Sequence Length: 608

• Applicable Applications for TDP1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TDP1 recombinant protein

TDP1, also known as tyrosyl-DNA phosphodiesterase 1, is involved in repairing stalled topoisomerase l-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase l and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical meditated DNA double-strand breaks. Recombinant human TDP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for TDP1 recombinant protein

Epigenomics (Transcription & Translation)

References

Jakobsen AK., et al, (2015) Exp. Mol. Pathol. 99:56-64.; Lebedeva NA., et al, (2015) Biosci. Rep. 35:e00230.

NCBI and Uniprot Product Information

• NCBI GI #: 20127586
• NCBI GeneID: 55775
• NCBI Accession #: NP_060789
• NCBI GenBank Nucleotide #: NM_018319.3
• UniProt Accession #: Q9NUW8; Q2HXX4; Q86TV8; Q96BK7; Q9NZM7; Q9NZM8
• Molecular Weight: 69.5kDa (617aa) 50-70kDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: tyrosyl-DNA phosphodiesterase 1 isoform a
• NCBI Official Synonym Full Names: tyrosyl-DNA phosphodiesterase 1
• NCBI Official Symbol: TDP1
• NCBI Protein Information: tyrosyl-DNA phosphodiesterase 1
• UniProt Protein Name: Tyrosyl-DNA phosphodiesterase 1
• Protein Family: Tyrosyl-DNA phosphodiesterase
• UniProt Gene Name: TDP1
• UniProt Synonym Gene Names: Tyr-DNA phosphodiesterase 1

NCBI Description

The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1). [provided by RefSeq, Aug 2016]

Uniprot Description

DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate.