Rabbit anti-Human, Mouse TDP1 Polyclonal Antibody | anti-TDP1 antibody

TDP1, ID (TDP1, Tyrosyl-DNA phosphodiesterase 1) (FITC)

Cat. Num. AAA6354626

• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TDP1; Polyclonal Antibody; ID (TDP1; Tyrosyl-DNA phosphodiesterase 1) (FITC); Tyrosyl-DNA phosphodiesterase 1; anti-TDP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TDP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TDP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-239 amino acids from the Central region of human TDP1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TDP1 Antibody (Center) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the TDP1 antibody detected the TDP1 protein (arrow).)

Related Product Information for anti-TDP1 antibody

The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1). While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform.

Product Categories/Family for anti-TDP1 antibody

Antibodies; Enzymes; Phosphodiesterase

References

Dexheimer, T.S., et al. Nucleic Acids Res. 38(7):2444-2452(2010). Chiang, S.C., et al. Cell Cycle 9(3):588-595(2010). Das, B.B., et al. EMBO J. 28(23):3667-3680(2009). Zhou, T., et al. DNA Repair (Amst.) 8(8):901-911(2009). Hoskins, J.M., et al. Pharmacogenomics 10(7):1139-1146(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 57242805
• NCBI GeneID: 55775
• NCBI Accession #: NP_001008744.1
• NCBI GenBank Nucleotide #: NM_001008744.1
• UniProt Accession #: Q9NUW8; Q2HXX4; Q86TV8; Q96BK7; Q9NZM7; Q9NZM8
• Molecular Weight: 41,630 Da
• NCBI Official Full Name: tyrosyl-DNA phosphodiesterase 1
• NCBI Official Synonym Full Names: tyrosyl-DNA phosphodiesterase 1
• NCBI Official Symbol: TDP1
• NCBI Protein Information: tyrosyl-DNA phosphodiesterase 1; tyr-DNA phosphodiesterase 1
• UniProt Protein Name: Tyrosyl-DNA phosphodiesterase 1
• Protein Family: Tyrosyl-DNA phosphodiesterase
• UniProt Gene Name: TDP1
• UniProt Synonym Gene Names: Tyr-DNA phosphodiesterase 1
• UniProt Entry Name: TYDP1_HUMAN

NCBI Description

The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1). While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

TDP1: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate. Monomer. Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus. Belongs to the tyrosyl-DNA phosphodiesterase family.

Protein type: Phosphodiesterase; EC 3.1.4.-; DNA repair, damage

Chromosomal Location of Human Ortholog: 14q32.11

Cellular Component: cytoplasm; nucleus

Molecular Function: tyrosyl-DNA phosphodiesterase activity; protein binding; double-stranded DNA binding; exonuclease activity; single-stranded DNA binding

Biological Process: single strand break repair; double-strand break repair; DNA repair

Disease: Spinocerebellar Ataxia, Autosomal Recessive, With Axonal Neuropathy