CTSL recombinant protein

CTSL, 18-333 aa, Human, His-tag, E Coli

Cat. Num. AAA206542

• Gene Names: CTSL; MEP; CATL; CTSL1
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: CTSL; 18-333 aa; Human; His-tag; E Coli; Cathepsin L1 isofrorm 1; CATL; CTSL1; MEP; CTSL recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl (pH 8.0) containing 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSTLTFDHS LEAQWTKWKA MHNRLYGMNE EGWRRAVWEK NMKMIELHNQ EYREGKHSFT MAMNAFGDMT SEEFRQVMNG FQNRKPRKGK VFQEPLFYEA PRSVDWREKG YVTPVKNQGQ CGSCWAFSAT GALEGQMFRK TGRLISLSEQ NLVDCSGPQG NEGCNGGLMD YAFQYVQDNG GLDSEESYPY EATEESCKYN PKYSVANDTG FVDIPKQEKA LMKAVATVGP ISVAIDAGHE SFLFYKEGIY FEPDCSSEDM DHGVLVVGYG FESTESDNNK YWLVKNSWGE EWGMGGYVKM AKDRRNHCGI ASAASYPTV
• Sequence Length: 333

• Applicable Applications for CTSL recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CTSL recombinant protein

CTSL, also known as cathepsin L1 isoform 1, is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. Recombinant Human CTSL protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for CTSL recombinant protein

Neuroscience

References

Bauer Y., et al. (2011) Hypertension. 57 (4):795-801; Wei DH., et al. (2013) Int J Mol Med. 31 (2):400-6

NCBI and Uniprot Product Information

• NCBI GI #: 384081594
• NCBI GeneID: 1514
• NCBI Accession #: NP_001244901
• NCBI GenBank Nucleotide #: NM_001257972.1
• UniProt Accession #: P07711; Q6IAV1; Q96QJ0
• Molecular Weight: 38.3kDa (339 aa) confirmed by MALDI-TOF
• NCBI Official Full Name: cathepsin L1 isoform 1 preproprotein
• NCBI Official Synonym Full Names: cathepsin L
• NCBI Official Symbol: CTSL
• NCBI Official Synonym Symbols: MEP; CATL; CTSL1
• NCBI Protein Information: cathepsin L1
• UniProt Protein Name: Cathepsin L1
• UniProt Gene Name: CTSL
• UniProt Synonym Gene Names: CTSL1; MEP

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

Important for the overall degradation of proteins in lysosomes.