SFRP4 recombinant protein

SFRP4, 19-346aa, Human, His-tag, Baculovirus

Cat. Num. AAA206477

• Gene Names: SFRP4; PYL; FRP-4; FRPHE; FRZB-2; sFRP-4
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: SFRP4; 19-346aa; Human; His-tag; Baculovirus; Secreted frizzled-related protein 4; FRP-4; FRPHE; PYL; sFRP-4; SFRP4 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPVRGAPCE AVRIPMCRHM PWNITRMPNH LHHSTQENAI LAIEQYEELV DVNCSAVLRF FLCAMYAPIC TLEFLHDPIK PCKSVCQRAR DDCEPLMKMY NHSWPESLAC DELPVYDRGV CISPEAIVTD LPEDVKWIDI TPDMMVQERP LDVDCKRLSP DRCKCKKVKP TLATYLSKNY SYVIHAKIKA VQRSGCNEVT TVVDVKEIFK SSSPIPRTQV PLITNSSCQC PHILPHQDVL IMCYEWRSRM MLLENCLVEK WRDQLSKRSI QWEERLQEQR RTVQDKKKTA GRTSRSNPPK PKGKPPAPKP ASPKKNIKTR SAQKRTNPKR VHHHHHH
• Sequence Length: 346

• Applicable Applications for SFRP4 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for SFRP4 recombinant protein

SFRP4, also known as secreted frizzled-related protein4, is a family of vertebrate proteins which contain homology to the ligand-binding domain of the Frizzled family of transmembrane receptors. It is expressed in brain, kidney, lung, ovary, prostate, mammary gland and endometrium. This protein act as soluble modulators of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations. It is able to bind Wnt proteins and Frizzled receptors in the extracellular compartment. Recombinant human SFRP4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for SFRP4 recombinant protein

Stem Cell

References

Jones SE., et al. (2002) Bioessays 24:811-820.; Berndt T., et al. (2003) J Clin Invest. 112:785-794.

NCBI and Uniprot Product Information

• NCBI GI #: 170784838
• NCBI GeneID: 6424
• NCBI Accession #: NP_003005
• NCBI GenBank Nucleotide #: NM_003014.3
• Molecular Weight: 38.9kDa (337aa) 40-57KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: secreted frizzled-related protein 4
• NCBI Official Synonym Full Names: secreted frizzled related protein 4
• NCBI Official Symbol: SFRP4
• NCBI Official Synonym Symbols: PYL; FRP-4; FRPHE; FRZB-2; sFRP-4
• NCBI Protein Information: secreted frizzled-related protein 4
• UniProt Protein Name: Secreted frizzled-related protein 4
• Protein Family: Secreted frizzled-related protein
• UniProt Gene Name: SFRP4
• UniProt Synonym Gene Names: FRPHE; sFRP-4; FrpHE

NCBI Description

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008]

Uniprot Description

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types (). SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake (PubMed:12952927).