Ctsa recombinant protein

Ctsa, 24-474aa, Mouse, His tag, Baculovirus

Cat. Num. AAA206220

• Gene Names: Ctsa; PPCA; Ppgb; AU019505
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Ctsa; 24-474aa; Mouse; His tag; Baculovirus; lysosomal protective protein isoform b; Cathepsin A; AU019505; PPCA; Ppgb; Ctsa recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: APDQDEIDCL PGLAKQPSFR QYSGYLRASD SKHFHYWFVE SQNDPKNSPV VLWLNGGPGC SSLDGLLTEH GPFLIQPDGV TLEYNPYAWN LIANVLYIES PAGVGFSYSD DKMYVTNDTE VAENNYEALK DFFRLFPEYK DNKLFLTGES YAGIYIPTLA VLVMQDPSMN LQGLAVGNGL ASYEQNDNSL VYFAYYHGLL GNRLWTSLQT HCCAQNKCNF YDNKDPECVN NLLEVSRIVG KSGLNIYNLY APCAGGVPGR HRYEDTLVVQ DFGNIFTRLP LKRRFPEALM RSGDKVRLDP PCTNTTAPSN YLNNPYVRKA LHIPESLPRW DMCNFLVNLQ YRRLYQSMNS QYLKLLSSQK YQILLYNGDV DMACNFMGDE WFVDSLNQKM EVQRRPWLVD YGESGEQVAG FVKECSHITF LTIKGAGHMV PTDKPRAAFT MFSRFLNKEP YVEHHHHHH
• Sequence Length: 474

• Applicable Applications for Ctsa recombinant protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Ctsa recombinant protein

CTSA, also known as lysosomal protective protein isoform b, is a component of the lysosomal multienzyme complex along with beta-galactosidase and sialidase Neu1. It is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities. Recombinant mouse CTSA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Ctsa recombinant protein

Signal Transduction; Metabolism

References

Seyrantepe V., et al. (2008) Circulation. 117:1973-1981.; Hiraiwa M., et al. (1999) Cell Mol Life Sci. 56:894-907.

NCBI and Uniprot Product Information

• NCBI GI #: 84042523
• NCBI GeneID: 19025
• NCBI Accession #: NP_001033581
• NCBI GenBank Nucleotide #: NM_001038492.2
• UniProt Accession #: P16675; Q8VEF6
• Molecular Weight: 52.4kDa (459aa) 50-70kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: lysosomal protective protein isoform b preproprotein
• NCBI Official Synonym Full Names: cathepsin A
• NCBI Official Symbol: Ctsa
• NCBI Official Synonym Symbols: PPCA; Ppgb; AU019505
• NCBI Protein Information: lysosomal protective protein
• UniProt Protein Name: Lysosomal protective protein
• Protein Family: Lysosomal protective protein
• UniProt Gene Name: Ctsa
• UniProt Synonym Gene Names: Ppgb; PPCA

NCBI Description

This gene encodes a glycoprotein with deamidase, esterase and carboxypeptidase activities. The encoded protein associates with and provides a protective function to the lysosomal enzymes beta-galactosidase and neuraminidase. Deficiency of the related gene in humans results in galactosialidosis. The proprotein is processed into two shorter chains. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.