Human Fibroblast Growth Factor 9 (FGF9) ELISA Kit | FGF9 elisa kit

Human Fibroblast Growth Factor 9 (FGF9) ELISA Kit

Cat. Num. AAA453530

• Gene Names: FGF9; GAF; FGF-9; SYNS3; HBFG-9; HBGF-9
• Reactivity: Human
• Synonyms: Fibroblast Growth Factor 9 (FGF9); Human Fibroblast Growth Factor 9 (FGF9) ELISA Kit; GAF; HBFG9; Glia-Activating Factor; FGF9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of FGF9. No significant cross-reactivity or interference between FGF9 and analogues was observed.
• Sequence Length: 208

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.064ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FGF9 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FGF9 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF9 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FGF9 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FGF9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FGF9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FGF9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FGF9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503707
• NCBI GeneID: 2254
• NCBI Accession #: NP_002001.1
• NCBI GenBank Nucleotide #: NM_002010.2
• UniProt Accession #: P31371; Q3SY32; A8K427
• Molecular Weight: 23,441 Da
• NCBI Official Full Name: fibroblast growth factor 9
• NCBI Official Synonym Full Names: fibroblast growth factor 9
• NCBI Official Symbol: FGF9
• NCBI Official Synonym Symbols: GAF; FGF-9; SYNS3; HBFG-9; HBGF-9
• NCBI Protein Information: fibroblast growth factor 9
• UniProt Protein Name: Fibroblast growth factor 9
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF9
• UniProt Synonym Gene Names: FGF-9; GAF; HBGF-9

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.