CA12 active protein

CA12, 25-301aa, Human, His tag, Baculovirus (Bioactivity Validated)

Cat. Num. AAA205850

• Gene Names: CA12; CAXII; CA-XII; T18816; HsT18816
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: CA12; 25-301aa; Human; His tag; Baculovirus (Bioactivity Validated); Carbonic anhydrase 12 isoform 1; CAXII; HsT18816; CA12 active protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: APVNGSKWTY FGPDGENSWS KKYPSCGGLL QSPIDLHSDI LQYDASLTPL EFQGYNLSAN KQFLLTNNGH SVKLNLPSDM HIQGLQSRYS ATQLHLHWGN PNDPHGSEHT VSGQHFAAEL HIVHYNSDLY PDASTASNKS EGLAVLAVLI EMGSFNPSYD KIFSHLQHVK YKGQEAFVPG FNIEELLPER TAEYYRYRGS LTTPPCNPTV LWTVFRNPVQ ISQEQLLALE TALYCTHMDD PSPREMINNF RQVQKFDERL VYTSFSQVQV CTAAGLSHHH HHH
• Sequence Length: 354

• Applicable Applications for CA12 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CA12 active protein

CA12, also known as Carbonic anhydrase 12, is an enzyme that in humans is encoded by the CA12 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Recombinant human CA12, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CA12 active protein

Cancer

References

Tureci O. et al. (1998) Proc Natl Acad Sci U S A. 95 (13):7608-13.; Kyllonen MS. et al. (2003) J Histochem Cytochem. 51 (9):1217-24.

NCBI and Uniprot Product Information

• NCBI GI #: 4502515
• NCBI GeneID: 771
• NCBI Accession #: NP_001209
• NCBI GenBank Nucleotide #: NM_001218.4
• UniProt Accession #: O43570; Q53YE5; Q9BWG2; B2RE24
• Molecular Weight: 31.94kDa (283aa) (28-40kDa in SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: carbonic anhydrase 12 isoform 1
• NCBI Official Synonym Full Names: carbonic anhydrase 12
• NCBI Official Symbol: CA12
• NCBI Official Synonym Symbols: CAXII; CA-XII; T18816; HsT18816
• NCBI Protein Information: carbonic anhydrase 12
• UniProt Protein Name: Carbonic anhydrase 12
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA12
• UniProt Synonym Gene Names: CA-XII

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014]

Uniprot Description

Reversible hydration of carbon dioxide.