EPO1L recombinant protein

EPO1L, 24-468aa, Human, His tag, E Coli

Cat. Num. AAA205679

• Gene Names: ERO1A; ERO1L; ERO1-L; ERO1LA; Ero1alpha; ERO1-alpha; ERO1-L-alpha
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: EPO1L; 24-468aa; Human; His tag; E Coli; ERO1-like protein alpha; ERO1-alpha; ERO1A; EPO1L recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSEEQPPET AAQRCFCQVS GYLDDCTCDV ETIDRFNNYR LFPRLQKLLE SDYFRYYKVN LKRPCPFWND ISQCGRRDCA VKPCQSDEVP DGIKSASYKY SEEANNLIEE CEQAERLGAV DESLSEETQK AVLQWTKHDD SSDNFCEADD IQSPEAEYVD LLLNPERYTG YKGPDAWKIW NVIYEENCFK PQTIKRPLNP LASGQGTSEE NTFYSWLEGL CVEKRAFYRL ISGLHASINV HLSARYLLQE TWLEKKWGHN ITEFQQRFDG ILTEGEGPRR LKNLYFLYLI ELRALSKVLP FFERPDFQLF TGNKIQDEEN KMLLLEILHE IKSFPLHFDE NSFFAGDKKE AHKLKEDFRL HFRNISRIMD CVGCFKCRLW GKLQTQGLGT ALKILFSEKL IANMPESGPS YEFHLTRQEI VSLFNAFGRI STSVKELENF RNLLQNIH
• Sequence Length: 468

• Applicable Applications for EPO1L recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for EPO1L recombinant protein

ERO1-like protein alpha, also known as EPO1L, is an essential oxidoreductase that oxidizes proteins and is required for the folding of immunoglobulins. EPO1L covalently binds with PDI (protein disulfide-isomerase) and together they produce disulfide bonds between proteins in the endoplasmic reticulum. EPO1L is stimulated by hypoxia, suggesting that it is regulated through the HIF (hypoxia inducible transcrip-tion factor) pathway. EPO1L is ubiquitously expressed at low levels but expressed at high levels in upper digestive tract and esophagus. Recombinant human EPO1L protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for EPO1L recombinant protein

Signal Transduction

References

Otsu M., et al. (2006) Antioxid. Redox Signal. 8: 274-282.; Gess B., et al. (2003) J Biochem. 270: 2228-2235.;

NCBI and Uniprot Product Information

• NCBI GI #: 7657069
• NCBI GeneID: 30001
• NCBI Accession #: NP_055399
• NCBI GenBank Nucleotide #: NM_014584.2
• UniProt Accession #: Q96HE7; Q7LD45; Q9P1Q9; Q9UKV6; A8K9X4; A8MYW1
• Molecular Weight: 54.4 kDa (468aa)
• NCBI Official Full Name: ERO1-like protein alpha
• NCBI Official Synonym Full Names: endoplasmic reticulum oxidoreductase 1 alpha
• NCBI Official Symbol: ERO1A
• NCBI Official Synonym Symbols: ERO1L; ERO1-L; ERO1LA; Ero1alpha; ERO1-alpha; ERO1-L-alpha
• NCBI Protein Information: ERO1-like protein alpha
• UniProt Protein Name: ERO1-like protein alpha
• UniProt Gene Name: ERO1A
• UniProt Synonym Gene Names: ERO1-L; ERO1-L-alpha

Uniprot Description

Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1.