Rabbit anti-Human ERO1A Polyclonal Antibody | anti-ERO1A antibody

ERO1A Antibody

Cat. Num. AAA7045930

• Gene Names: ERO1A; ERO1L; ERO1-L; ERO1LA; Ero1alpha; ERO1-alpha; ERO1-L-alpha
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: ERO1A; Polyclonal Antibody; ERO1A Antibody; ERO1-like protein alpha; ERO1-L; ERO1-L-alpha; Endoplasmic oxidoreductin-1-like protein; Endoplasmic reticulum oxidoreductase alpha; anti-ERO1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 468

• Applicable Applications for anti-ERO1A antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Species: Human
• Immunogen: Recombinant human ERO1-like protein alpha protein (24-260AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-79942 / sc-98354
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: ERO1L antibody at 1.25ug/mlLane 1 : A431 whole cell lysateLane 2 : 293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 54 kDaObserved band size: 54,25 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human small intestine tissue using MBS7045930 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human pancreatic tissue using MBS7045930 at dilution 1:100)

Related Product Information for anti-ERO1A antibody

Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1.

NCBI and Uniprot Product Information

• NCBI GI #: 7657069
• NCBI GeneID: 30001
• NCBI Accession #: NP_055399.1
• NCBI GenBank Nucleotide #: NM_014584.2
• UniProt Accession #: Q96HE7; Q7LD45; Q9P1Q9; Q9UKV6; A8K9X4; A8MYW1
• Molecular Weight: 54,393 Da
• NCBI Official Full Name: ERO1-like protein alpha
• NCBI Official Synonym Full Names: endoplasmic reticulum oxidoreductase 1 alpha
• NCBI Official Symbol: ERO1A
• NCBI Official Synonym Symbols: ERO1L; ERO1-L; ERO1LA; Ero1alpha; ERO1-alpha; ERO1-L-alpha
• NCBI Protein Information: ERO1-like protein alpha
• UniProt Protein Name: ERO1-like protein alpha
• Protein Family: ERO1-like protein
• UniProt Gene Name: ERO1A
• UniProt Synonym Gene Names: ERO1-L; ERO1-L-alpha
• UniProt Entry Name: ERO1A_HUMAN

Uniprot Description

ERO1L: Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. Predominantly monomer. May function both as a monomer and a homodimer. Interacts with PDILT. Stimulated by hypoxia; suggesting that it is regulated via the HIF-pathway. Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum. Enzyme activity is tightly regulated to prevent the accumulation of reactive oxygen species in the endoplasmic reticulum. Reversibly down-regulated by the formation of disulfide bonds between the active site Cys-94 and Cys-131, and between Cys- 99 and Cys-104. Glutathione may be required to regulate its activity in the endoplasmic reticulum. Belongs to the EROs family.

Protein type: Secreted, signal peptide; EC 1.8.4.-; Secreted; Oxidoreductase

Chromosomal Location of Human Ortholog: 14q22.1

Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; intracellular membrane-bound organelle; membrane

Molecular Function: disulfide oxidoreductase activity; oxidoreductase activity; protein binding

Biological Process: chaperone cofactor-dependent protein folding; protein folding; protein modification process; release of sequestered calcium ion into cytosol; response to reactive oxygen species; response to temperature stimulus