Mouse Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit | TGFb3 elisa kit

Mouse Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit

Cat. Num. AAA455369

• Gene Names: Tgfb3; Tgfb-3; TGF-beta-3
• Reactivity: Mouse
• Synonyms: Transforming Growth Factor Beta 3 (TGFb3); Mouse Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit; TGF-B3; LAP; Latency-associated peptide; TGFb3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of TGFb2. No significant cross-reactivity or interference between TGFb2 and analogues was observed.
• Sequence Length: 412

• Samples: Bovine Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 5.8pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TGFb2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TGFb2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TGFb3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of TGFb2 in bovine serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGFb2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TGFb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TGFb2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TGFb2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 225637540
• NCBI GeneID: 21809
• NCBI Accession #: NP_033394.2
• NCBI GenBank Nucleotide #: NM_009368.3
• UniProt Accession #: Q3TRQ9; P17125
• Molecular Weight: 40,423 Da
• NCBI Official Full Name: transforming growth factor beta-3
• NCBI Official Synonym Full Names: transforming growth factor, beta 3
• NCBI Official Symbol: Tgfb3
• NCBI Official Synonym Symbols: Tgfb-3; TGF-beta-3
• NCBI Protein Information: transforming growth factor beta-3
• UniProt Protein Name: Putative uncharacterized protein
• Protein Family: Transforming growth factor
• UniProt Gene Name: Tgfb3

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. This protein is involved in embryogenesis and cell differentiation, and may play a role in wound healing. Homozygous knockout mice for this gene exhibit cleft palate, delayed pulmonary development and neonatal death. [provided by RefSeq, Aug 2016]