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SDS-Page

DCAF7 recombinant protein

DCAF7, 19-295aa, Human, His tag, E Coli (Denatured)

Gene Names
DCAF7; AN11; HAN11; WDR68; SWAN-1
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
DCAF7; 19-295aa; Human; His tag; E Coli (Denatured); DDB1-and CuL4-associated factor 7; AN11; HAN11; SWAN-1; WDR68; DCAF7 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 1mM DTT
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MVYAMNWSVR PDKRFRLALG SFVEEYNNKV QLVGLDEESS EFICRNTFDH PYPTTKLMWI PDTKGVYPDL LATSGDYLRV WRVGETETRL ECLLNNNKNS DFCAPLTSFD WNEVDPYLLG TSSIDTTCTI WGLETGQVLG RVNLVSGHVK TQLIAHDKEV YDIAFSRAGG GRDMFASVGA DGSVRMFDLR HLEHSTIIYE DPQHHPLLRL CWNKQDPNYL ATMAMDGMEV VILDVRVPCT PVARLNNHRA CVNGIAWAPH SSCHICTAAD DHQALIWD
Sequence Length
342
Applicable Applications for DCAF7 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for DCAF7 recombinant protein
DCAF7, also known as DDB1-and CuL4-associated factor, involved in craniofacial development. This protein acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches. This protein associates with DIAPH1 and controls GLI1 transcriptional activity. This protein could be involved in normal and disease skin development. Recombinant human DCAF7 protein, fused to His-tag at N-terminus, was expressed in E Coli and denatured using detergent during conventional chromatography purification process.
Product Categories/Family for DCAF7 recombinant protein
References
Morita K., et al. (2006) J. Dermatol. Sci. 44:11-20; Jin J., et al. (2006) Mol. Cell. 23:709-721

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33.6 kDa (298aa)
NCBI Official Full Name
DDB1- and CUL4-associated factor 7
NCBI Official Synonym Full Names
DDB1 and CUL4 associated factor 7
NCBI Official Symbol
DCAF7
NCBI Official Synonym Symbols
AN11; HAN11; WDR68; SWAN-1
NCBI Protein Information
DDB1- and CUL4-associated factor 7
UniProt Protein Name
DDB1- and CUL4-associated factor 7
UniProt Gene Name
DCAF7
UniProt Synonym Gene Names
HAN11; WDR68

NCBI Description

This gene encodes a protein with multiple WD40 repeats which facilitate protein-protein interactions and thereby enable the assembly of multiprotein complexes. This protein has been shown to function as a scaffold protein for protein complexes involved in kinase signaling. This highly conserved gene is present in eukaryotic plants, fungi, and animals. The ortholog of this gene was first identified in plants as a key regulator of anthocyanin biosynthesis and flower pigmentation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

Involved in craniofacial development. Acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches (). Associates with DIAPH1 and controls GLI1 transcriptional activity. Could be involved in normal and disease skin development. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.

Research Articles on DCAF7

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Product Notes

The DCAF7 dcaf7 (Catalog #AAA204129) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's DCAF7 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the DCAF7 dcaf7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MVYAMNWSVR PDKRFRLALG SFVEEYNNKV QLVGLDEESS EFICRNTFDH PYPTTKLMWI PDTKGVYPDL LATSGDYLRV WRVGETETRL ECLLNNNKNS DFCAPLTSFD WNEVDPYLLG TSSIDTTCTI WGLETGQVLG RVNLVSGHVK TQLIAHDKEV YDIAFSRAGG GRDMFASVGA DGSVRMFDLR HLEHSTIIYE DPQHHPLLRL CWNKQDPNYL ATMAMDGMEV VILDVRVPCT PVARLNNHRA CVNGIAWAPH SSCHICTAAD DHQALIWD. It is sometimes possible for the material contained within the vial of "DCAF7, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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