SDS-Page
EIF5A2 recombinant protein
EIF5A2, 1-153aa, Human, His tag, E Coli
Gene Names
EIF5A2; EIF-5A2; eIF5AII
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
EIF5A2; 1-153aa; Human; His tag; E Coli; Eukaryotic translation initiation factor 5A2; eIF 5A 2; eIF 5A2; eIF5AII; Eukaryotic initiation factor 5A isoform 2; eIF-4D; EIF5A 2; MGC124092; MGC124093; EIF5A2 recombinant protein
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MADEIDFTTG DAGASSTYPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDYQ LICIQDGYLS LLTETGEVRE DLKLPEGELG KEIEGKYNAG EDVQVSVMCA MSEEYAVAIK PCK
Sequence Length
153
Applicable Applications for EIF5A2 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for EIF5A2 recombinant protein
EIF5A2, a member of eukaryotic initiation factor 5A subfamily, is an essential protein tightly linked to cellular polyamine homeostasis. The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond during the initial stage of protein synthesis. It seems to be the only eukaryotic protein to have a hypusine residue, which is a post-translational modification of a lysine by the addition of a butylamino group. Recombinant human EIF5A2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for EIF5A2 recombinant protein
References
Guan XY., et al. (2010) Hepatology. 51 (4):1255-63.; Luk JM., et al. (2009) Int J Cancer. 127 (4):968-76.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18.9 kDa (173aa), confirmed by MALDI-TOF
NCBI Official Full Name
eukaryotic translation initiation factor 5A-2
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 5A2
NCBI Official Symbol
EIF5A2
NCBI Official Synonym Symbols
EIF-5A2; eIF5AII
NCBI Protein Information
eukaryotic translation initiation factor 5A-2
UniProt Protein Name
Eukaryotic translation initiation factor 5A-2
UniProt Gene Name
EIF5A2
UniProt Synonym Gene Names
eIF-5A-2; eIF-5A2
Uniprot Description
mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation ().
Research Articles on EIF5A2
Similar Products
Product Notes
The EIF5A2 eif5a2 (Catalog #AAA203694) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's EIF5A2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the EIF5A2 eif5a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MADEIDFTTG DAGASSTYPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDYQ LICIQDGYLS LLTETGEVRE DLKLPEGELG KEIEGKYNAG EDVQVSVMCA MSEEYAVAIK PCK. It is sometimes possible for the material contained within the vial of "EIF5A2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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