Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TOPBP1 antibody.)
Rabbit anti-Human TOPBP1 Polyclonal Antibody | anti-TOPBP1 antibody
TOPBP1 Antibody
Immunohistochemistry: 1:50 - 1:200
Immunofluorescence: 1:50 - 1:200
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TOPBP1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TOPBP1 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cell using TOPBP1 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cell using TOPBP1 antibody. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands. [provided by RefSeq, Jul 2008]
Uniprot Description
TOPBP1: a DNA-binding protein and scaffolding protein that is required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR. Contains 7 BRCT domains, which are characteristic of proteins involved in cell cycle checkpoint control in response to DNA damage. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase. Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Up-regulated during the S phase of the cell cycle. Up-regulated by E2F1 and interferon.
Protein type: DNA replication
Chromosomal Location of Human Ortholog: 3q22.1
Cellular Component: male germ cell nucleus; spindle pole; Golgi apparatus; centrosome; PML body; condensed nuclear chromosome; cytoplasm; chromosome; nucleus; actin cytoskeleton
Molecular Function: protein C-terminus binding; identical protein binding; protein binding; DNA binding
Biological Process: response to ionizing radiation; DNA repair; DNA metabolic process; response to DNA damage stimulus
Research Articles on TOPBP1
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Product Notes
The TOPBP1 topbp1 (Catalog #AAA9411774) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TOPBP1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TOPBP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200. Researchers should empirically determine the suitability of the TOPBP1 topbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TOPBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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