RRM2 recombinant protein

RRM2, 1-389aa, Human, His tag, E Coli

Cat. Num. AAA203601

• Gene Names: RRM2; R2; RR2; RR2M
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: RRM2; 1-389aa; Human; His tag; E Coli; Ribonucleoside-diphosphate reductase subunit M2; R2; RR2; RR2M; Ribonucleoside-diphosphate reductase subunit M2 Ribonucleotide reductase M2 subunit; Ribonucleotide reductase small subunit; ; RRM2 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 0.1M NaCl
• Concentration: 1.0 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MLSLRVPLAP ITDPQQLQLS PLKGLSLVDK ENTPPALSGT RVLASKTARR IFQEPTEPKT KAAAPGVEDE PLLRENPRRF VIFPIEYHDI WQMYKKAEAS FWTAEEVDLS KDIQHWESLK PEERYFISHV LAFFAASDGI VNENLVERFS QEVQITEARC FYGFQIAMEN IHSEMYSLLI DTYIKDPKER EFLFNAIETM PCVKKKADWA LRWIGDKEAT YGERVVAFAA VEGIFFSGSF ASIFWLKKRG LMPGLTFSNE LISRDEGLHC DFACLMFKHL VHKPSEERVR EIIINAVRIE QEFLTEALPV KLIGMNCTLM KQYIEFVADR LMLELGFSKV FRVENPFDFM ENISLEGKTN FFEKRVGEYQ RMGVMSSPTE NSFTLDADF
• Sequence Length: 389

• Applicable Applications for RRM2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RRM2 recombinant protein

RRM2, also known as ribonucleotide reductase M2, is an enzyme that catalyzes the formation of deoxyribonucleotides from ribonucleotides. This protein plays a critical role in regulating the total rate of DNA synthesis so that DNA to cell mass is maintained at a constant ratio during cell division and DNA repair. Recombinant human RRM2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques

Product Categories/Family for RRM2 recombinant protein

Enzymes & Proteases

References

Torrents E, et al. (2002) J Mol Evol. 55 (2):138-52; Herrick J,et al. (2007) Mol Microbiol. 63 (1):22-34;

NCBI and Uniprot Product Information

• NCBI GI #: 4557845
• NCBI GeneID: 6241
• NCBI Accession #: NP_001025
• NCBI GenBank Nucleotide #: NM_001034.3
• UniProt Accession #: P31350; Q5WRU7; B2R9B5; J3KP43
• Molecular Weight: 47.0 kDa (409aa) confirmed by MALDI-TOF
• NCBI Official Full Name: ribonucleoside-diphosphate reductase subunit M2 isoform 2
• NCBI Official Synonym Full Names: ribonucleotide reductase regulatory subunit M2
• NCBI Official Symbol: RRM2
• NCBI Official Synonym Symbols: R2; RR2; RR2M
• NCBI Protein Information: ribonucleoside-diphosphate reductase subunit M2
• UniProt Protein Name: Ribonucleoside-diphosphate reductase subunit M2
• Protein Family: Ribonucleoside-diphosphate reductase
• UniProt Gene Name: RRM2
• UniProt Synonym Gene Names: RR2

NCBI Description

This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X. [provided by RefSeq, Sep 2009]

Uniprot Description

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.