Goat ATP1B1 Polyclonal Antibody | anti-ATP1B1 antibody

Goat Polyclonal to Human ATP1B1

Cat. Num. AAA244138

• Gene Names: ATP1B1; ATP1B
• Reactivity: Gibbon, Chimpanzee, Dog, Gorilla, Hamster, Horse, Human, Mouse, Orangutan, Rat; Predicted Reactivity: Monkey, Guinea pig, Pig (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: ATP1B1; Polyclonal Antibody; Goat Polyclonal to Human ATP1B1; Anti-ATP1B1 Antibody (Internal) IHC-plus; Adenosinetriphosphatase; ATP1B; Atpb1; Human ATP1B1; anti-ATP1B1 antibody

• Host: Goat
• Reactivity: Gibbon, Chimpanzee, Dog, Gorilla, Hamster, Horse, Human, Mouse, Orangutan, Rat; Predicted Reactivity: Monkey, Guinea pig, Pig (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human ATP1B1. This antibody is expected to recognize both reported isoforms (NP_001668.1; NP_001001787.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 303

• Applicable Applications for anti-ATP1B1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:128000), IHC-P (5 ug/ml), WB (0.1 - 0.3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 48kDa band observed in Rat Retina lysates and 50kDa in Mouse and Rat Kidney lysates (calculated MW of 35.1kDa according to Human NP_001668.1, 35.2kDa according to Mouse N...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-KTEISFRPNDPKSYE from an internal region of human ATP1B1 (NP_001668.1; NP_001001787.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Dog, Horse (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: ATP1B1 antibody was raised against synthetic peptide C-KTEISFRPNDPKSYE from an internal region of human ATP1B1 (NP_001668.1; NP_001001787.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Dog, Horse (100%); Marmoset, Elephant, Panda, Pig, Guinea pig (93%); Sheep, Bovine, Rabbit (87%); Chicken (80%).
• Antigen Modification: Internal
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-ATP1B1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(ATP1B1 antibody (0.1 ug/ml) staining of Rat Retina lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-ATP1B1 antibody

Family: Transporter; Subfamily: ATPase - P type; beta subunit

NCBI and Uniprot Product Information

• NCBI GI #: 4502277
• NCBI GeneID: 481
• NCBI Accession #: NP_001668.1
• NCBI GenBank Nucleotide #: NM_001677.3
• UniProt Accession #: P05026; Q5TGZ3
• Molecular Weight: 34,893 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit beta-1
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, beta 1 polypeptide
• NCBI Official Symbol: ATP1B1
• NCBI Official Synonym Symbols: ATP1B
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit beta-1; adenosinetriphosphatase; sodium pump subunit beta-1; Na, K-ATPase beta-1 polypeptide; Beta 1-subunit of Na(+),K(+)-ATPase; sodium/potassium-dependent ATPase beta-1 subunit; sodium/potassium-transporting ATPase beta-1 chain; sodium-potassium ATPase subunit beta 1 (non-catalytic)
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit beta-1
• UniProt Gene Name: ATP1B1
• UniProt Synonym Gene Names: ATP1B
• UniProt Entry Name: AT1B1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. [provided by RefSeq, Mar 2010]

Uniprot Description

ATP1B1: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Belongs to the X(+)/potassium ATPases subunit beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: basolateral plasma membrane; apical plasma membrane; plasma membrane; caveola; sodium:potassium-exchanging ATPase complex; sarcolemma; vesicle

Molecular Function: protein binding; ATPase activator activity; sodium:potassium-exchanging ATPase activity; ATPase binding

Biological Process: cellular calcium ion homeostasis; cellular sodium ion homeostasis; potassium ion import; protein stabilization; regulation of gene expression; positive regulation of ATPase activity; response to hypoxia; blood coagulation; cell adhesion; leukocyte migration; transmembrane transport; cellular potassium ion homeostasis; cardiac muscle contraction

Disease: Hypertension, Essential