Poly U Binding Splicing Factor 60kDa Recombinant Protein | PUF60 recombinant protein

Recombinant Poly U Binding Splicing Factor 60kDa (PUF60)

Cat. Num. AAA2034111

• Gene Names: PUF60; FIR; VRJS; RoBPI; SIAHBP1
• Reactivity: Human
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Poly U Binding Splicing Factor 60kDa; Recombinant Poly U Binding Splicing Factor 60kDa (PUF60); FIR; SIAHBP1; RoBPI; Fuse Binding Protein Interacting Repressor; Siah Binding Protein 1; Pyrimidine Tract Binding Splicing Factor; Ro Ribonucleoprotein Binding Protein 1; PUF60 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: >90%
• Sequence Length: 499

• Applicable Applications for PUF60 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Fragment: Asn226~Gln549
• Tag: Prokaryotic expression
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 402794208
• NCBI GeneID: 22827
• NCBI Accession #: NP_001258029.1
• NCBI GenBank Nucleotide #: NM_001271100.1
• Molecular Weight: 64.6kDa
• NCBI Official Full Name: poly(U)-binding-splicing factor PUF60 isoform h
• NCBI Official Synonym Full Names: poly(U) binding splicing factor 60KDa
• NCBI Official Symbol: PUF60
• NCBI Official Synonym Symbols: FIR; VRJS; RoBPI; SIAHBP1
• NCBI Protein Information: poly(U)-binding-splicing factor PUF60
• UniProt Protein Name: Poly(U)-binding-splicing factor PUF60
• Protein Family: Poly(U)-binding-splicing factor
• UniProt Gene Name: PUF60
• UniProt Synonym Gene Names: FIR; ROBPI; SIAHBP1; FBP-interacting repressor; RoBP1; Siah-BP1
• UniProt Entry Name: PUF60_HUMAN

NCBI Description

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Uniprot Description

FIR: fuse-binding protein-interacting repressor. Interacts with (and may activate) Ro RNPs. Forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. Two alternatively spliced isoforms have been described.

Protein type: RNA splicing; Transcription, coactivator/corepressor; RNA-binding; Spliceosome

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cell junction; cyclin-dependent protein kinase activating kinase holoenzyme complex; nucleoplasm; ribonucleoprotein complex

Molecular Function: DNA binding; identical protein binding; nucleotide binding; protein binding

Biological Process: apoptosis; mRNA processing; regulation of transcription, DNA-dependent; RNA splicing; transcription, DNA-dependent

Disease: Verheij Syndrome