Mouse CD209 antigen-like protein A ELISA Kit | Cd209a elisa kit

Mouse CD209 antigen-like protein A ELISA Kit

Cat. Num. AAA288018

• Gene Names: Cd209a; CIRE; CD209; CDSIGN; Dcsign; SIGNR5; DC-SIGN; SIGN-R1; DC-SIGN1
• Reactivity: Mouse
• Synonyms: CD209 antigen-like protein A; Mouse CD209 antigen-like protein A ELISA Kit; Cd209a elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse CD209A. No significant cross-reactivity or interference was observed.
• Sequence Length: 238

• Assay Type: Sandwich
• Detection Range: 15.6-1000 pg/mL
• Sensitivity: 8.2 pg/mL
• Intra-Assay CV: <=3.5%
• Inter-Assay CV: <=7.1%
• Recovery: 105%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Cd209a elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD209A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CD209A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CD209A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CD209A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18875404
• NCBI GeneID: 170786
• NCBI Accession #: NP_573501.1
• NCBI GenBank Nucleotide #: NM_133238.5
• UniProt Accession #: Q91ZX1; Q3TAT9; Q91ZW5
• Molecular Weight: 24,055 Da
• NCBI Official Full Name: CD209 antigen-like protein A
• NCBI Official Synonym Full Names: CD209a antigen
• NCBI Official Symbol: Cd209a
• NCBI Official Synonym Symbols: CIRE; CD209; CDSIGN; Dcsign; SIGNR5; DC-SIGN; SIGN-R1; DC-SIGN1
• NCBI Protein Information: CD209 antigen-like protein A
• UniProt Protein Name: CD209 antigen-like protein A
• Protein Family: CD209 antigen-like protein
• UniProt Gene Name: Cd209a
• UniProt Synonym Gene Names: Cire; DC-SIGN
• UniProt Entry Name: C209A_MOUSE

Uniprot Description

CD209: Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri- mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA. Homotetramer. Binds to many viral surface glycoproteins such as HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus envelope glycoproteins, cytomegalovirus gB, HCV E2 and dengue virus major envelope protein E. Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes. 13 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: cell surface; integral to membrane; membrane

Molecular Function: calcium-dependent protein binding; carbohydrate binding; mannose binding; metal ion binding; viral receptor activity

Biological Process: endocytosis; entry of virus into host cell; regulation of blood coagulation; regulation of gene expression; regulation of T cell proliferation