Western Blot (WB)
(Western Blot;Sample: Human Jurkat cell lysate;Primary Ab: 1ug/ml Rabbit Anti-Mouse EPRS AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Rabbit Glutamyl Prolyl tRNA Synthetase Polyclonal Antibody | anti-EPRS antibody
Polyclonal Antibody to Glutamyl Prolyl tRNA Synthetase (EPRS)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot;Sample: Human Jurkat cell lysate;Primary Ab: 1ug/ml Rabbit Anti-Mouse EPRS AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot (WB)
(Western Blot;Sample: Human Jurkat cell lysate;Primary Ab: 1ug/ml Rabbit Anti-Mouse EPRS AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot (WB)
(Western Blot:Sample: Recombinant EPRS, Mouse.)
Western Blot (WB)
(Western Blot:Sample: Recombinant EPRS, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded stomach tissue)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded stomach tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Mouse Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Mouse Kidney Tissue)
NCBI and Uniprot Product Information
Uniprot Description
EPRS: Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43. Interacts with DUS2L.
Protein type: Aminoacyl-tRNA synthetase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 6.1.1.15; EC 6.1.1.17; Ligase; Translation; Translation regulation
Chromosomal Location of Human Ortholog: 1|1 H5
Cellular Component: aminoacyl-tRNA synthetase multienzyme complex; cytoplasm; cytosol; intracellular ribonucleoprotein complex; membrane
Molecular Function: aminoacyl-tRNA ligase activity; ATP binding; catalytic activity; glutamate-tRNA ligase activity; GTPase binding; identical protein binding; ligase activity; metal ion binding; nucleotide binding; proline-tRNA ligase activity; protein homodimerization activity; RNA binding; zinc ion binding
Biological Process: cellular response to interferon-gamma; glutamyl-tRNA aminoacylation; metabolic process; negative regulation of translation; prolyl-tRNA aminoacylation; regulation of translation; translation; tRNA aminoacylation; tRNA aminoacylation for protein translation
Research Articles on EPRS
Similar Products
Product Notes
The EPRS eprs (Catalog #AAA2033779) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glutamyl Prolyl tRNA Synthetase (EPRS) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Glutamyl Prolyl tRNA Synthetase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the EPRS eprs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutamyl Prolyl tRNA Synthetase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
