Glutamyl Prolyl tRNA Synthetase Recombinant Protein | EPRS recombinant protein

Recombinant Glutamyl Prolyl tRNA Synthetase (EPRS)

Cat. Num. AAA2034292

• Gene Names: EPRS; EARS; PARS; QARS; QPRS; PIG32; GLUPRORS
• Reactivity: Mouse
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Glutamyl Prolyl tRNA Synthetase; Recombinant Glutamyl Prolyl tRNA Synthetase (EPRS); PARS; EARS; PIG32; QPRS; QARS; Bifunctional Aminoacyl-TRNA Synthetase; Glutamate tRNA Ligase; Proline tRNA Ligase; Cell proliferation-inducing gene 32 protein; EPRS recombinant protein

• Host: E Coli
• Reactivity: Mouse
• Purity/Purification: >90%
• Sequence Length: 1512

• Applicable Applications for EPRS recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Fragment: Gly1007~Tyr1512
• Tag: Two N-terminal Tags, His-tag and T7-tag
• Expression System: Prokaryotic Expression
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 223460490
• NCBI GeneID: 2058
• Molecular Weight: 61.3kDa
• NCBI Official Full Name: Glutamyl-prolyl-tRNA synthetase
• NCBI Official Synonym Full Names: glutamyl-prolyl-tRNA synthetase
• NCBI Official Symbol: EPRS
• NCBI Official Synonym Symbols: EARS; PARS; QARS; QPRS; PIG32; GLUPRORS
• NCBI Protein Information: bifunctional glutamate/proline--tRNA ligase
• UniProt Protein Name: Bifunctional glutamate/proline--tRNA ligase
• UniProt Gene Name: EPRS
• UniProt Synonym Gene Names: GLNS; PARS; QARS; QPRS; GluRS
• UniProt Entry Name: SYEP_HUMAN

NCBI Description

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

EPRS: Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43. Interacts with DUS2L.

Protein type: EC 6.1.1.17; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Ligase; EC 6.1.1.15; Translation

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: aminoacyl-tRNA synthetase multienzyme complex; cytoplasm; cytosol; membrane; ribonucleoprotein complex

Molecular Function: ATP binding; glutamate-tRNA ligase activity; GTPase binding; proline-tRNA ligase activity; protein binding

Biological Process: gene expression; glutamyl-tRNA aminoacylation; negative regulation of translation; prolyl-tRNA aminoacylation; protein complex assembly; selenium metabolic process; tRNA aminoacylation for protein translation; tRNA modification; tRNA processing