Insulin Like Growth Factor Binding Protein 2 Recombinant Protein | IGFBP2 recombinant protein

Recombinant Insulin Like Growth Factor Binding Protein 2 (IGFBP2)

Cat. Num. AAA2033481

• Gene Names: IGFBP2; IBP2; IGF-BP53
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Insulin Like Growth Factor Binding Protein 2; Recombinant Insulin Like Growth Factor Binding Protein 2 (IGFBP2); IBP2; IGF-BP53; IGFBP2 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: DNGDDHSEGGLVE NHVDSTMNML GGGGSAGRKP LKSGMKELAV FREKVTEQHRQMGKGGKHHL GLEEPKKLRP PPARTPCQQE LDQVLERIST MRLPDERGPLEHLYSLHIPN CDKHGLYNLK QCKMSLNGQR GECWCVNPNT GKLIQGAPTIRGDPECHLFY NEQQEARGVH TQRMQ
• Sequence Length: 159

• Applicable Applications for IGFBP2 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 23.7kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Asp148~Gln325
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 929524276
• NCBI GeneID: 3485
• NCBI Accession #: NP_001300921.1
• NCBI GenBank Nucleotide #: NM_001313992.1
• Molecular Weight: 34,814 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 2 isoform c
• NCBI Official Synonym Full Names: insulin like growth factor binding protein 2
• NCBI Official Symbol: IGFBP2
• NCBI Official Synonym Symbols: IBP2; IGF-BP53
• NCBI Protein Information: insulin-like growth factor-binding protein 2
• UniProt Protein Name: Insulin-like growth factor-binding protein 2
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP2
• UniProt Synonym Gene Names: BP2; IBP2; IBP-2; IGF-binding protein 2; IGFBP-2
• UniProt Entry Name: IBP2_HUMAN

NCBI Description

The protein encoded by this gene is one of six similar proteins that bind insulin-like growth factors I and II (IGF-I and IGF-II). The encoded protein can be secreted into the bloodstream, where it binds IGF-I and IGF-II with high affinity, or it can remain intracellular, interacting with many different ligands. High expression levels of this protein promote the growth of several types of tumors and may be predictive of the chances of recovery of the patient. Several transcript variants, one encoding a secreted isoform and the others encoding nonsecreted isoforms, have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

IGFBP2: Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Binds IGF2 more than IGF1.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: apical plasma membrane; cytoplasmic vesicle; extracellular region; extracellular space

Molecular Function: insulin-like growth factor I binding; insulin-like growth factor II binding; protein binding

Biological Process: aging; cellular protein metabolic process; cellular response to hormone stimulus; female pregnancy; positive regulation of activated T cell proliferation; regulation of cell growth; regulation of insulin-like growth factor receptor signaling pathway; response to drug; response to estradiol stimulus; response to glucocorticoid stimulus; response to lithium ion; response to mechanical stimulus; response to nutrient; response to retinoic acid; signal transduction