Interferon Regulatory Factor 8 Recombinant Protein | IRF8 recombinant protein

Recombinant Interferon Regulatory Factor 8 (IRF8)

Cat. Num. AAA2033464

• Gene Names: IRF8; ICSBP; IRF-8; ICSBP1; IMD32A; IMD32B; H-ICSBP
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Interferon Regulatory Factor 8; Recombinant Interferon Regulatory Factor 8 (IRF8); H-ICSBP; ICS-BP; ICSBP1; Interferon Consensus Sequence-Binding Protein 1; IRF8 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: MCDRNGGRRL RQWLIEQIDS SMYPGLIWEN DEKTMFRIPW KHAGKQDYNQ EVDASIFKAW AVFKGKFKEG DKAEPATWKT RLRCALNKSP DFEEVTDRSQ LDISEPYKVY RIVPEEEQKC KLGVAPAGCM SEVPEMECGR SEIEELIKEP SVDEYMGMTK RSPSPPEACR SQILPDWWVQ QPSAGLPLVT GYAAYDTHHS AFSQMVISFY YGGKLVGQAT TTCLEGCRLS LSQPGLPKLY GPDGLEPVCF PTADTIPSER QRQVTRKLFG HLERGVLLHS NRKGVFVKRL CQGRVFCSGNAVVCKGRPNK LERDEVVQVF DTNQFIR
• Sequence Length: 426

• Applicable Applications for IRF8 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 40.9kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Arg327
• Organism Species: Mouse
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4504567
• NCBI GeneID: 3394
• NCBI Accession #: NP_002154.1
• NCBI GenBank Nucleotide #: NM_002163.2
• Molecular Weight: 48,356 Da
• NCBI Official Full Name: interferon regulatory factor 8
• NCBI Official Synonym Full Names: interferon regulatory factor 8
• NCBI Official Symbol: IRF8
• NCBI Official Synonym Symbols: ICSBP; IRF-8; ICSBP1; IMD32A; IMD32B; H-ICSBP
• NCBI Protein Information: interferon regulatory factor 8
• UniProt Protein Name: Interferon regulatory factor 8
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: IRF8
• UniProt Synonym Gene Names: ICSBP1; IRF-8; H-ICSBP; ICSBP
• UniProt Entry Name: IRF8_HUMAN

NCBI Description

Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. [provided by RefSeq, Jul 2008]

Uniprot Description

IRF8: Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Interacts (via C-terminus) with TRIM21 (via C-terminus). Interacts with COPS2. By IFNG/IFN-gamma. Predominantly expressed in lymphoid tissues. Belongs to the IRF family.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 16q24.1

Cellular Component: cytosol; nucleoplasm

Molecular Function: protein binding; RNA polymerase II transcription factor activity, enhancer binding

Biological Process: cytokine and chemokine mediated signaling pathway; defense response to bacterium; defense response to protozoan; immune response; myeloid cell differentiation; negative regulation of transcription from RNA polymerase II promoter; phagocytosis; positive regulation of interferon-gamma production; positive regulation of interleukin-12 production; transcription, DNA-dependent

Disease: Immunodeficiency 32a; Monocyte And Dendritic Cell Deficiency, Autosomal Recessive