Immunohistochemistry (IHC)
(Immunochemical staining of mouse ASGR1 in mouse liver with rabbit polyclonal antibody (1 ug/mL, formalin-fixed paraffin embedded sections).)
Rabbit anti-Mouse Asialoglycoprotein Receptor Polyclonal Antibody | anti-ASGR1 antibody
Anti-Asialoglycoprotein Receptor Antibody, Rabbit Polyclonal
Gene Names
Asgr1; Asgr; ASGPR1; Asgr-1
Reactivity
Mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
Asialoglycoprotein Receptor; Polyclonal Antibody; Anti-Asialoglycoprotein Receptor Antibody; Rabbit Polyclonal; ASGPR1/ASGR1 Antibody; Rabbit PAb; Antigen Affinity Purified; asialoglycoprotein receptor 1; Anti-ASGPR1 Antibody; Anti-Asgr Antibody; Anti-Asgr-1 Antibody; Anti-HL-1 Antibody; anti-ASGR1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse ASGPR1/ASGR1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ASGR1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
IP: 0.2-1uL/mg of lysate
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
IP: 0.2-1uL/mg of lysate
Immunogen
Recombinant Mouse ASGPR1/ASGR1 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse ASGPR1/ASGR1 (rM ASGPR1/ASGR1). ASGPR1/ASGR1 specific IgG was purified by Mouse ASGPR1/ASGR1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of mouse ASGR1 in mouse liver with rabbit polyclonal antibody (1 ug/mL, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse ASGR1 in mouse liver with rabbit polyclonal antibody (1 ug/mL, formalin-fixed paraffin embedded sections).)
Immunoprecipitation (IP)
(Mouse ASGR1 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 0.5 uL anti-Mouse ASGR1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse ASGR1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDa Observed band size: 42 kDa)
Immunoprecipitation (IP)
(Mouse ASGR1 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 0.5 uL anti-Mouse ASGR1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse ASGR1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDa Observed band size: 42 kDa)
Western Blot (WB)
(Anti-ASGR1 rabbit polyclonal antibody at 1:500 dilution Lane A: NIH3T3 whole cell lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:36 kDa)
Western Blot (WB)
(Anti-ASGR1 rabbit polyclonal antibody at 1:500 dilution Lane A: NIH3T3 whole cell lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:36 kDa)
Related Product Information for anti-ASGR1 antibody
The asialoglycoprotein receptor (ASGPR), an endocytotic cell surface receptor expressed by hepatocytes, is triggered by triantennary binding to galactose residues of macromolecules such as asialoorosomucoid (ASOR). ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The research regarded that ASGPR1 could be targeted for anti-hepatitis B virus (HBV) drug development.
References
Yang J, et al. (2006) Antisense oligonucleotides targeted against asialoglycoprotein receptor 1 block human hepatitis B virus replication. J Viral Hepat. 13(3): 158-65.Li Y, et al. (2008) Targeted delivery of macromolecular drugs: asialoglycoprotein receptor (ASGPR) expression by selected hepatoma cell lines used in antiviral drug development. Curr Drug Deliv. 5(4): 299-302.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,604 Da
NCBI Official Full Name
asialoglycoprotein receptor 1
NCBI Official Synonym Full Names
asialoglycoprotein receptor 1
NCBI Official Symbol
Asgr1
NCBI Official Synonym Symbols
Asgr; ASGPR1; Asgr-1
NCBI Protein Information
asialoglycoprotein receptor 1; HL-1; mHL-1; ASGPR 1; ASGP-R 1; hepatic lectin 1
UniProt Protein Name
Asialoglycoprotein receptor 1
Protein Family
UniProt Gene Name
Asgr1
UniProt Synonym Gene Names
Asgr-1; ASGP-R 1; ASGPR 1; HL-1; mHL-1
UniProt Entry Name
ASGR1_MOUSE
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Product Notes
The ASGR1 asgr1 (Catalog #AAA8110932) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Asialoglycoprotein Receptor Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Asialoglycoprotein Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:1000 ELISA: 1:5000-1:10,000 IHC-P: 1:500-1:2000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the ASGR1 asgr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Asialoglycoprotein Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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