ATP Binding Cassette Transporter C11 Recombinant Protein | ABCC11 recombinant protein

Recombinant ATP Binding Cassette Transporter C11 (ABCC11)

Cat. Num. AAA2033162

• Gene Names: ABCC11; WW; EWWD; MRP8
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: ATP Binding Cassette Transporter C11; Recombinant ATP Binding Cassette Transporter C11 (ABCC11); ABC-C11; MRP8; EWWD; Multidrug resistance-associated protein 8; ABCC11 recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: IIFQDYHMKYRDNTPTVLHG INLTIRGHEV VGIVGRTGSG KSSLGMALFR LVEPMAGRILIDGVDICSIG LEDLRSKLSV IPQDPVLLSG TIRFNLDPFD RHTDQQIWDALERTFLTKAI SKFPKKLHTD VVENGGNFSV GERQLLCIAR AVLRNSKIILIDEATASIDM ETDTLIQRTI REAFQGCTVL VIAHRVTTVL NCDHILVMGNGKVVEFDRPE VLRKKPGSLF AALMATATSS LR
• Sequence Length: 1344

• Applicable Applications for ABCC11 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 30.5kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Ile1141~Arg1382
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 19851882
• NCBI GeneID: 85320
• Molecular Weight: 150,093 Da
• NCBI Official Full Name: ATP-binding cassette protein C11 isoform A
• NCBI Official Synonym Full Names: ATP binding cassette subfamily C member 11
• NCBI Official Symbol: ABCC11
• NCBI Official Synonym Symbols: WW; EWWD; MRP8
• NCBI Protein Information: ATP-binding cassette sub-family C member 11
• UniProt Protein Name: ATP-binding cassette sub-family C member 11
• Protein Family: ABC transporter C family
• UniProt Gene Name: ABCC11
• UniProt Synonym Gene Names: MRP8
• UniProt Entry Name: ABCCB_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This ABC full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. The product of this gene participates in physiological processes involving bile acids, conjugated steroids, and cyclic nucleotides. In addition, a SNP in this gene is responsible for determination of human earwax type. This gene and family member ABCC12 are determined to be derived by duplication and are both localized to chromosome 16q12.1. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCC11: Participates in physiological processes involving bile acids, conjugated steroids and cyclic nucleotides. Enhances the cellular extrusion of cAMP and cGMP. Stimulates the ATP-dependent uptake of a range of physiological and synthetic lipophilic anions, including the glutathione S-conjugates leukotriene C4 and dinitrophenyl S-glutathione, steroid sulfates such as dehydroepiandrosterone 3-sulfate (DHEAS) and estrone 3-sulfate, glucuronides such as estradiol 17-beta-D-glucuronide (E(2)17betaG), the monoanionic bile acids glycocholate and taurocholate, and methotrexate. Probably functions to secrete earwax. Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ABC family; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 16q12.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: ATP binding; ATPase activity, coupled to transmembrane movement of substances; organic anion transmembrane transporter activity; purine nucleotide transmembrane transporter activity

Biological Process: metabolic process; organic anion transport; purine nucleotide transport; transmembrane transport

Disease: Apocrine Gland Secretion, Variation In