Myosin Heavy Chain 7B, Cardiac Muscle Beta Recombinant Protein | MYH7B recombinant protein

Recombinant Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B)

Cat. Num. AAA2033114

• Gene Names: MYH7B; MHC14; MYH14
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Myosin Heavy Chain 7B; Cardiac Muscle Beta; Recombinant Myosin Heavy Chain 7B; Cardiac Muscle Beta (MYH7B); MYH14; MHC14; Antigen MLAA-21; Myosin cardiac muscle beta chain; MYH7B recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: NA VKNWSWMKLF FKMKPLLRSAQAEEELAALR AELRGLRGAL AAAEAKRQEL EETHVSITQE KNDLALQLQAEQDNLADAEE RCHLLIKSKV QLEGKVKELS ERLEDEEEVN ADLAARRRKLEDECTELKKD IDDLKLTLAK AEKEKQATEN KVKNLTEEMA ALDESVARLTKEKKALQEAH QQALGDLQAE EDRVSALTKA KLRLEQQVED LECSLEQEKKLRMDTERAKR KLEGDLKLTQ ESVADAAQDK QQLEEKLKKK DSELSQLSLRVEDEQL
• Sequence Length: 1983

• Applicable Applications for MYH7B recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 61.8kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Asn829~Leu1106
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and GST-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 153217512
• NCBI GeneID: 57644
• Molecular Weight: 20,273 Da
• NCBI Official Full Name: Myosin, heavy chain 7B, cardiac muscle, beta
• NCBI Official Synonym Full Names: myosin, heavy chain 7B, cardiac muscle, beta
• NCBI Official Symbol: MYH7B
• NCBI Official Synonym Symbols: MHC14; MYH14
• NCBI Protein Information: myosin-7B
• UniProt Protein Name: Myosin-7B
• Protein Family: Myosin
• UniProt Gene Name: MYH7B
• UniProt Synonym Gene Names: KIAA1512
• UniProt Entry Name: MYH7B_HUMAN

NCBI Description

The myosin II molecule is a multi-subunit complex consisting of two heavy chains and four light chains. This gene encodes a heavy chain of myosin II, which is a member of the motor-domain superfamily. The heavy chain includes a globular motor domain, which catalyzes ATP hydrolysis and interacts with actin, and a tail domain in which heptad repeat sequences promote dimerization by interacting to form a rod-like alpha-helical coiled coil. This heavy chain subunit is a slow-twitch myosin. Alternatively spliced transcript variants have been found, but the full-length nature of these variants is not determined. [provided by RefSeq, Mar 2010]

Uniprot Description

MYH7B: Involved in muscle contraction. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Contractile; Motor

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: membrane

Molecular Function: actin binding; ATP binding; motor activity; protein binding

Biological Process: metabolic process