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SDS-Page

2',3'-Cyclic Nucleotide 3'-Phosphodiesterase Recombinant Protein | CNP recombinant protein

Recombinant 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase (CNP)

Gene Names
Cnp; CNPF; CNPI; Cnp1; CNPII
Applications
SDS-Page, Western Blot
Purity
> 97%
Synonyms
2'; 3'-Cyclic Nucleotide 3'-Phosphodiesterase; Recombinant 2'; 3'-Cyclic Nucleotide 3'-Phosphodiesterase (CNP); 2; 3CNP; 3-CNP; CNPCNP1; Cyclic-CMP Phosphodiesterase; 3'-Cyclic AMP Phosphodiesterase; CNPase; 2':3'-CNMP-3'-ase; CNP recombinant protein
Ordering
For Research Use Only!
Host
E. coli
Purity/Purification
> 97%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 600ug/mL (varies by lot)
Sequence
LPLYF GWFLTKKSSE TLRKAGQVFL EELGNHKAFK KELRQFVPGD EPREKMDLVT YFGKRPPGVL HCTTKFCDYG KAPGAEEYAQ QDVLKKSYSK AFTLTISALF VTPKTTGARV ELSEQQLQLW PSDVDKLSPT DNLPRGSRAH ITLGCAADVE AVQTGLDLLE ILRQEKGGSR GEEVGELSRG KLYSLGNGRW MLTLAKNMEV RAIFTGYYGK GKPVPTQGSR KGGALQSCTII
Sequence Length
420
Applicable Applications for CNP recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB)

(May be suitable for use in other assays to be determined by the end user.)
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Leu186~Ile421
Tags
N-terminal His Tag
Subcellular Location
Membrane
Traits
Freeze-dried powder
Predicted isoelectric point
9.3
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 29.8kDa
Accurate Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
2',3'-cyclic-nucleotide 3'-phosphodiesterase
NCBI Official Synonym Full Names
2',3'-cyclic nucleotide 3' phosphodiesterase
NCBI Official Symbol
Cnp
NCBI Official Synonym Symbols
CNPF; CNPI; Cnp1; CNPII
NCBI Protein Information
2',3'-cyclic-nucleotide 3'-phosphodiesterase
UniProt Protein Name
2',3'-cyclic-nucleotide 3'-phosphodiesterase
UniProt Gene Name
Cnp
UniProt Synonym Gene Names
Cnp1; CNP; CNPase
UniProt Entry Name
CN37_RAT

NCBI Description

microtubule-binding protein that promotes microtubule assembly and may regulate cellular distribution of microtubules [RGD, Feb 2006]

Uniprot Description

CNP: May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. Exists as monomers and homodimers. Belongs to the cyclic nucleotide phosphodiesterase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Phosphodiesterase; EC 3.1.4.37; Cell development/differentiation

Cellular Component: cell projection; cytoplasm; extracellular space; melanosome; membrane; microtubule; microvillus; mitochondrial inner membrane; mitochondrial outer membrane; myelin sheath; nucleoplasm; nucleus; perinuclear region of cytoplasm; plasma membrane; pseudopodium

Molecular Function: 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity; cyclic nucleotide binding; RNA binding

Biological Process: adult locomotory behavior; aging; axonogenesis; cyclic nucleotide catabolic process; forebrain development; microtubule cytoskeleton organization and biogenesis; oligodendrocyte differentiation; regulation of mitochondrial membrane permeability; response to lipopolysaccharide; response to toxin; substantia nigra development

Research Articles on CNP

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Product Notes

The CNP cnp (Catalog #AAA2033046) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB) (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the CNP cnp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LPLYF GWFLTKKSSE TLRKAGQVFL EELGNHKAFK KELRQFVPGD EPREKMDLVT YFGKRPPGVL HCTTKFCDYG KAPGAEEYAQ QDVLKKSYSK AFTLTISALF VTPKTTGARV ELSEQQLQLW PSDVDKLSPT DNLPRGSRAH ITLGCAADVE AVQTGLDLLE ILRQEKGGSR GEEVGELSRG KLYSLGNGRW MLTLAKNMEV RAIFTGYYGK GKPVPTQGSR KGGALQSCTI I. It is sometimes possible for the material contained within the vial of "2',3'-Cyclic Nucleotide 3'-Phosphodiesterase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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