Rabbit anti-Human TNPO1 Polyclonal Antibody | anti-Tnpo1 antibody

TNPO1, NT (TNPO1, KPNB2, MIP1, TRN, Transportin-1, Importin beta-2, Karyopherin beta-2, M9 region interaction protein)

Cat. Num. AAA648790

• Gene Names: Tnpo1; MIP; TRN; IPO2; MIP1; Kpnb2; AU021749; D13Ertd688e
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TNPO1; Polyclonal Antibody; NT (TNPO1; KPNB2; MIP1; TRN; Transportin-1; Importin beta-2; Karyopherin beta-2; M9 region interaction protein); Anti -TNPO1; anti-Tnpo1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-Tnpo1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: TNPO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45~75 amino acids from the N-terminal region of human TNPO1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TNPO1 Antibody (N-term) in 293 cell line lysates (35ug/lane). TNPO1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with TNPO1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TNPO1 Antibody (N-term) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-Tnpo1 antibody

TNPO1 comprises the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins.

Product Categories/Family for anti-Tnpo1 antibody

Antibodies; Abs to Transport Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 115385968
• NCBI GeneID: 238799
• NCBI Accession #: NP_848831.2
• NCBI GenBank Nucleotide #: NM_178716.3
• UniProt Accession #: Q8BFY9; Q8C0S3; Q8K2E7
• Molecular Weight: 102,357 Da
• NCBI Official Full Name: transportin-1 isoform 1
• NCBI Official Synonym Full Names: transportin 1
• NCBI Official Symbol: Tnpo1
• NCBI Official Synonym Symbols: MIP; TRN; IPO2; MIP1; Kpnb2; AU021749; D13Ertd688e
• NCBI Protein Information: transportin-1; importin beta-2; karyopherin beta-2; karyopherin (importin) beta 2
• UniProt Protein Name: Transportin-1
• UniProt Gene Name: Tnpo1
• UniProt Synonym Gene Names: Kpnb2
• UniProt Entry Name: TNPO1_MOUSE

Uniprot Description

Function: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus

By similarity. Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A

By similarity. In vitro, mediates nuclear import of SRP19

By similarity and H2A, H2B, H3 and H4 histones. Mediates nuclear import of ADAR/ADAR1 in a RanGTP-dependent manner

By similarity. Ref.3

Subunit structure: Interacts with H2A, H2B, H3 and H4 histones

By similarity. Binds HNRPA1, HNRPA2, HNRNPDL, RPL23A, RPS7, RPL5, RAN and SRP19. Interacts with ADAR/ADAR1 (via DRBM 3 domain). Interacts with SNAI1 (via zinc fingers); the interaction mediates SNAI1 nuclear import. Interacts with SNAI2 (via zinc fingers)

By similarity. Ref.3

Subcellular location: Cytoplasm

By similarity. Nucleus

By similarity.

Sequence similarities: Belongs to the importin beta family.Contains 8 HEAT repeats.Contains 1 importin N-terminal domain.

Sequence caution: The sequence BAC26696.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC27029.1 differs from that shown. Reason: Erroneous initiation.