Transmembrane Protein 27 Recombinant Protein | TMEM27 recombinant protein

Recombinant Transmembrane Protein 27 (TMEM27)

Cat. Num. AAA2033009

• Gene Names: TMEM27; NX17; NX-17
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Transmembrane Protein 27; Recombinant Transmembrane Protein 27 (TMEM27); NX17; Collectrin; TMEM27 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: ELCRPD AENAFKVRLS IKAALGDKAY VWDTDEEYLFRAMVAFSMRK VPNREGTEIS HVLLCNVTQR VSFWFVVTDP LKNHTLPAAEVQSAIRMNRN RINSAFFLDD HTLEFLKIPS TLAPPMDPSV P
• Sequence Length: 222

• Applicable Applications for TMEM27 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 18.2kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Glu15~Pro141
• Organism Species: Rat
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119619300
• NCBI GeneID: 57393
• Molecular Weight: 25,235 Da
• NCBI Official Full Name: transmembrane protein 27
• NCBI Official Synonym Full Names: transmembrane protein 27
• NCBI Official Symbol: TMEM27
• NCBI Official Synonym Symbols: NX17; NX-17
• NCBI Protein Information: collectrin
• UniProt Protein Name: Collectrin
• Protein Family: Collectrin
• UniProt Gene Name: TMEM27
• UniProt Entry Name: TMM27_HUMAN

NCBI Description

This gene encodes a type 1 transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules. The encoded protein binds to amino acid transporters and regulates their expression on the plasma membrane. It also plays a role in controlling insulin exocytosis by regulating formation of the SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) complex in pancreatic beta cells. The extracellular domain of the encoded protein may be cleaved and shed from the plasma membrane specifically in pancreatic beta cells. [provided by RefSeq, Jun 2013]

Uniprot Description

TMEM27: Regulator of SNARE complex function. Stimulator of beta cell replication. Belongs to the TMEM27 family.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: Xp22

Cellular Component: brush border membrane; cytoplasm; integral to membrane

Molecular Function: metallopeptidase activity; peptidyl-dipeptidase activity; protein binding

Biological Process: positive regulation of amino acid transport; positive regulation of calcium ion-dependent exocytosis; proteolysis