SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
GFP recombinant protein
GFP, 1-238aa, Aequorea victoria, E Coli
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
GFP; 1-238aa; Aequorea victoria; E Coli; Green fluorescent protein; GFP recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK
Sequence Length
1-238
Applicable Applications for GFP recombinant protein
SDS-PAGE
Tag
Non-tagged.
Endotoxin Level
< 1.0 EU per 1 ug of protein (determined by LAL method)
Preparation and Storage
Can be stored at 2-8°C for 1 week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for GFP recombinant protein
GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant GFP protein was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for GFP recombinant protein
References
Millan MI, et al. (2009). Medicina. 69 (3):370-4.; Marshall J, et al. (1995). Neuron, 14 (2):211-5.;
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
26.8 kDa (238aa), confirmed by MALDI-TOF.
NCBI Official Full Name
green-fluorescent protein
UniProt Protein Name
Green fluorescent protein
Protein Family
UniProt Gene Name
GFP
Uniprot Description
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.
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Product Notes
The GFP gfp (Catalog #AAA203279) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GFP can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the GFP gfp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK. It is sometimes possible for the material contained within the vial of "GFP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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