Rabbit anti-Human SPAM1 Polyclonal Antibody | anti-SPAM1 antibody

SPAM1, CT (SPAM1, HYAL3, PH20, Hyaluronidase PH-20, Hyaluronoglucosaminidase PH-20, Sperm adhesion molecule 1, Sperm surface protein PH-20) (APC)

Cat. Num. AAA6350306

• Gene Names: SPAM1; HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SPAM1; Polyclonal Antibody; CT (SPAM1; HYAL3; PH20; Hyaluronidase PH-20; Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface protein PH-20) (APC); Sperm surface protein PH-20; anti-SPAM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 509

• Applicable Applications for anti-SPAM1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SPAM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 429-457 amino acids from the C-terminal region of human SPAM1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SPAM1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SPAM1 antibody detected the SPAM1 protein (arrow).)

Related Product Information for anti-SPAM1 antibody

Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-SPAM1 antibody

Antibodies; Enzymes; Glycosidase

References

Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Dunn, C.A., et al. BMC Genomics 6 (1), 47 (2005): Evans, E.A., et al. Reprod. Biol. Endocrinol. 1, 54 (2003): Cherr, G.N., et al. Matrix Biol. 20(8):515-525(2001). Csoka, A.B., et al. Matrix Biol. 20(8):499-508(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 23510418
• NCBI GeneID: 6677
• NCBI Accession #: NP_694859.1
• NCBI GenBank Nucleotide #: NP_694859.1
• UniProt Accession #: P38567
• NCBI Official Full Name: hyaluronidase PH-20 isoform 2
• NCBI Official Synonym Full Names: sperm adhesion molecule 1
• NCBI Official Symbol: SPAM1
• NCBI Official Synonym Symbols: HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n
• NCBI Protein Information: hyaluronidase PH-20
• UniProt Protein Name: Hyaluronidase PH-20
• Protein Family: Hyaluronidase
• UniProt Gene Name: SPAM1
• UniProt Synonym Gene Names: HYAL3; PH20; Hyal-PH20
• UniProt Entry Name: HYALP_HUMAN

NCBI Description

Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

SPAM1: Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. Belongs to the glycosyl hydrolase 56 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Glycan Metabolism - glycosaminoglycan degradation; Hydrolase; EC 3.2.1.35; Membrane protein, GPI anchor; Cell surface

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: plasma membrane

Molecular Function: hyalurononglucosaminidase activity

Biological Process: sperm-egg recognition; fusion of sperm to egg plasma membrane; binding of sperm to zona pellucida; single fertilization; carbohydrate metabolic process; cell adhesion; multicellular organism reproduction