Mouse anti-Human CD86 Monoclonal Antibody | anti-CD86 antibody

Anti-Hu CD86 Purified Low Endotoxin

Cat. Num. AAA1800447

• Gene Names: CD86; B70; B7-2; B7.2; LAB72; CD28LG2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot, Immunohistochemistry
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: CD86; Monoclonal Antibody; Anti-Hu CD86 Purified Low Endotoxin; CD86 molecule; B7-2; FUN-1; LAB72; anti-CD86 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: BU63
• Specificity: The mouse monoclonal antibody BU63 reacts with an extracellular epitope of CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 329

• Applicable Applications for anti-CD86 antibody: Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB), Immunohistochemistry (IHC) Frozen, Functional
• Application Notes: FC/FACS: QC tested
• Immunogen: B-lymphoblastoid cell line ARH 77
• Preparation and Storage: Store at 2-8 degree C.

Flow Cytometry (FC/FACS)

(Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD86 (BU63) purified antibody (low endotoxin, concentration in sample 3 ug/ml) GAM APC.)

Related Product Information for anti-CD86 antibody

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.

NCBI and Uniprot Product Information

• NCBI GI #: 317373339
• NCBI GeneID: 942
• UniProt Accession #: P42081
• NCBI Official Full Name: T-lymphocyte activation antigen CD86
• NCBI Official Synonym Full Names: CD86 molecule
• NCBI Official Symbol: CD86
• NCBI Official Synonym Symbols: B70; B7-2; B7.2; LAB72; CD28LG2
• NCBI Protein Information: T-lymphocyte activation antigen CD86
• UniProt Protein Name: T-lymphocyte activation antigen CD86
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: CD86
• UniProt Synonym Gene Names: CD28LG2
• UniProt Entry Name: CD86_HUMAN

NCBI Description

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Uniprot Description

CD86: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: cell surface; intracellular membrane-bound organelle; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; coreceptor activity; receptor activity; receptor binding

Biological Process: positive regulation of lymphotoxin A biosynthetic process; negative regulation of T cell anergy; T cell activation; viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of interleukin-2 biosynthetic process; myeloid dendritic cell differentiation; positive regulation of activated T cell proliferation; positive regulation of interleukin-4 biosynthetic process; positive regulation of T-helper 2 cell differentiation; cell-cell signaling; T cell proliferation during immune response; positive regulation of cell proliferation; response to yeast; defense response to virus; aging; response to drug; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; B cell activation; T cell costimulation; toll-like receptor signaling pathway; innate immune response; immune response